Microglia Markers

Microglia Markers

Microglia are the resident immune cells of the central nervous system (CNS). They originate from yolk sac progenitors during embryonic development and serve as the brain’s primary innate immune defense. In the healthy CNS, microglia continuously survey the microenvironment, contributing to synaptic pruning, neurogenesis, and tissue homeostasis. Upon injury, infection, or neurodegeneration, they become activated and undergo morphological and functional changes, including cytokine release, phagocytosis, and antigen presentation.

To support microglia research, Alomone Labs offers a wide range of high-quality rabbit and guinea pig polyclonal antibodies, including both conjugated and unconjugated formats. These antibodies are validated for use in multiple applications such as immunohistochemistry (IHC), immunofluorescence (IF), Western blotting (WB), and flow cytometry (FACS), enabling precise detection and characterization of microglial markers across experimental systems.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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90 Results
  • Anti-CD39 (extracellular)-FITC Antibody
    Anti-CD39 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CD39 Conjugated to the Fluorescent Dye FITC
    • Product Code: ANT-065-F
    • Short Description: A Rabbit Polyclonal Antibody to CD39 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Ectonucleoside Triphosphate Diphosphohydrolase 1, Ecto-ATP diphosphohydrolase 1, Lymphoid cell activation antigen, ENTPD1
  • Anti-CLEC7A/Dectin-1 (extracellular) Antibody
    Anti-CLEC7A/Dectin-1 (extracellular) Antibody
    A Rabbit Polyclonal antibody to CLEC7A/Dectin-1 (extracellular)
    • Product Code: ALR-062
    • Short Description: A Rabbit Polyclonal antibody to CLEC7A/Dectin-1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: C-Type Lectin Domain Family 7 Member A, Dendritic Cell-Associated C-Type Lectin 1, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, C-Type Lectin Superfamily Member 12, CLECSF12, CD369
  • Anti-CLEC7A/Dectin-1 (extracellular)-FITC Antibody
    Anti-CLEC7A/Dectin-1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CLEC7A (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ALR-062-F
    • Short Description: A Rabbit Polyclonal Antibody to CLEC7A (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: C-Type Lectin Domain Family 7 Member A, Dendritic Cell-Associated C-Type Lectin 1, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, C-Type Lectin Superfamily Member 12, CLECSF12, CD369, SCARE2
  • Anti-CX3CR1 (extracellular) Antibody
    Anti-CX3CR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CX3CR1 Receptor
    • Product Code: ACR-058
    • Short Description: A Rabbit Polyclonal Antibody to CX3CR1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1
  • Anti-CX3CR1 (extracellular)-APC Antibody
    Anti-CX3CR1 (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to CX3CR1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: ACR-058-APC
    • Short Description: A Rabbit Polyclonal Antibody to CX3CR1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1
  • Anti-CX3CR1 (extracellular)-FITC Antibody
    Anti-CX3CR1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CX3CR1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACR-058-F
    • Short Description: A Rabbit Polyclonal Antibody to CX3CR1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1
  • Anti-EMR1 (ADGRE1) (extracellular) Antibody
    Anti-EMR1 (ADGRE1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EMR1
    • Product Code: AER-051
    • Short Description: A Rabbit Polyclonal Antibody to EMR1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: EMR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480
  • Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody
    Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to EMR1 Conjugated to the Fluorescent Dye FITC
    • Product Code: AER-051-F
    • Short Description: A Rabbit Polyclonal Antibody to EMR1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: EMR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480
  • Anti-GPR109A/HCAR2 (extracellular) Antibody
    Anti-GPR109A/HCAR2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to GPR109A/HCAR2 (extracellular)
    • Product Code: AHR-012
    • Short Description: A Rabbit Polyclonal antibody to GPR109A/HCAR2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Hydroxycarboxylic Acid Receptor 2, Niacin Receptor 1, G-Protein Coupled Receptor HM74A, HM74A, Nicotinic acid receptor, PUMAG
  • Anti-GPR109A/HCAR2 (extracellular)-FITC Antibody
    Anti-GPR109A/HCAR2 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR109A/HCAR2 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AHR-012-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR109A/HCAR2 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Hydroxycarboxylic Acid Receptor 2, Niacin Receptor 1, G-Protein Coupled Receptor HM74A, HM74A, Nicotinic acid receptor, PUMAG
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