Immuno-Colocalization Updates

Alomone Labs has developed two innovative platforms for studying protein-protein interaction and/or protein co-localization in complex immunostaining or immunoprecipitation protocols:

ATTO-Conjugated Antibodies
These antibodies can be used in immunohistochemistry (IHC) and immunocytochemistry (ICC) with same species antibodies.

Save time and resources with ONE antibody.

Benefits of immunostaining with ATTO-conjugated antibodies:
Direct immuno-fluorescence
Secondary antibodies are not necessary
Ideal for immuno-colocalization with same species/host antibodies

Guinea Pig-Raised Antibodies
Antibodies raised in guinea pig can be used with any non-guinea pig second species antibodies (e.g. antibodies raised in mouse, rabbit, goat, etc.) in complex immunostaining or immunoprecipitation protocols. Our line of guinea pig raised antibodies can be combined with any of our rabbit-raised or ATTO-conjugated antibodies in complex immunostaining protocols.

The complete list of immuno-colocalization products.

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Immuno-Colocalization of TRPV4 and Aquaporin 2 in Mouse Kidney


Immunohistochemical staining of mouse kidney sections using Anti-TRPV4 Antibody (#ACC-034) and Anti-Aquaporin 2-ATTO-590 Antibody (#AQP-002-AO). TRPV4 staining (green) is detected in the tubules of the cortex and meddula (left panel). AQP2 immunoreactivity (red) is detected in the same areas (middle panel). Merged image shows complete overlap between TRPV4 and AQP2 staining. Adapted from Berrout, J. et al. (2012) J. Biol. Chem. 287, 8782. with permission of the American Society for Biochemistry and Molecular Biology.

Immuno-Colocalization of VGLUT2 and Neurokinin Receptor 1 in Rat DRG
Immuno-Colocalization of KCa3.1 and P2Y2 in Human Ovarian Carcinoma
Immuno-Colocalization of GluN1 and ASCT1 in Mouse Hippocampus
Immuno-Colocalization of GIRK2 and LRRK2 in Mouse Substantia Nigra
Immuno-Colocalization of GluN2A and SynGAP in Rat Cingulate Cortex
Immuno-Colocalization of Kir6.2 and BDNF in Mouse Hippocampus
Immuno-Colocalization of Aquaporin 4 and TrkB in Rat Cerebellum
Immuno-Colocalization of Synapsin-2 and GABA(A) α1 Receptor in Rat Striatum
Immuno-Colocalization of Slitrk1 and KV2.1 in Rat Parietal Cortex
Immuno-Colocalization of VAChT and p75NTR in Rat Medial Septum
Immuno-Colocalization of Plexin-A2 and p75NTR in Rat Brain
Immuno-Colocalization of Kir3.2 and KCNK2 in Rat Substantia Nigra
Immuno-Colocalization of VGAT and Gephyrin in Rat Spinal Cord
Immuno-Colocalization of Kir4.1 and α1-Syntrophin in Rat Fornix
Immuno-Colocalization of proNGF and Cannabinoid Receptor 1 in Mouse Hippocampus
Immuno-Colocalization of NET and NK1 in Rat Brain Stem
Immuno-Colocalization of GluR1 and PICK1 in Rat Hippocampus
Immuno-Colocalization of TRPM8 and TrkA in Rat DRG
Immuno-Colocalization of ASIC1 and ASIC3 in Rat DRG
Immuno-Colocalization of DPP6 and KV4.2 in Rat Hippocampus
Immuno-Colocalization of GluN2A and GluN2B in Mouse Deep Cerebellar Nucleus
Immuno-Colocalization of NaV1.8 and Synaptophysin in Rat DRG
Immuno-Colocalization of HCN4 and HCN2 in Mouse Thalamus
Immuno-Colocalization of KV1.3 and KV1.5 in Mouse Cerebellum
Immuno-Colocalization of TrkB and BDNF in Mouse Hippocampus
Immuno-Colocalization of GluN1 and Plexin-A1 in Rat Olfactory Bulb
Immuno-Colocalization of GluN1 and M4 Muscarinic Receptor in Mouse Parietal Cortex
Immuno-Colocalization of CaV1.2 and GABA(A) α1 Receptor in Rat Hippocampus
Immuno-Colocalization of TRPC6 and TRPC3 in Rat Cerebellum
Immuno-Colocalization of GluN1 and CALHM1 in Mouse Hippocampal CA1 Region
Immuno-Colocalization of ASIC1 and Parvalbumin in Rat Hippocampus
Immuno-Colocalization of Neurexin 1α and GABA(A) ε Receptor in Mouse Cerebellum
Immuno-Colocalization of mGluR1 and TRPC3 in Mouse Cerebellum
Immuno-Colocalization of PSD-95 and GluN2B in Rat Brain
Immuno-Colocalization of nAChRα4 and nAChRβ2 in Mouse Brain
Immuno-Colocalization of VMAT2 and TRPC3 in Rat Brain
Immuno-Colocalization of AT2 Receptor and VGLUT2 in Rat Supraoptic Hypothalamic Nucleus
Immuno-Colocalization of Aquaporin 2 and Neutrophil Gelatinase-Associated Lipocalin (Ngal) in Mouse Kidney
Immuno-Colocalization of Aquaporin 2 and TRPV4 in Rat Kidney
Immuno-Colocalization of PTH2R and VGLUT2 in Mouse Hippocampus
Tityustoxin-Kα-ATTO-594 Colocalizes with KV1.2 Channel and Parvalbumin in Mouse Cerebellum
Immuno-Colocalization of p75NTR and TrkA in Rat DRG
Immuno-Colocalization of CaV1.2 and GABA(A) α1 Receptor in Mouse Hippocampus
Immuno-Colocalization of VGLUT2 and P2X7 Receptor in Rat Spinal Cord
Immuno-Colocalization of KCa2.3 (SK3) and Aquaporin 2 in Mouse Kidney
Immuno-Colocalization of KV1.1 and KV1.2 in Mouse Cerebellum
Immuno-Colocalization of CaV1.2 and GABA(A) α1 Receptor in Rat Cerebellum
Immuno-Colocalization of TrkB in Mouse Cerebellum
Immuno-Colocalization of Melatonin Receptor Type 2 and AT2 Receptor in Rat Brain
Immuno-Colocalization of Melatonin Receptor 2 and SCN2A (NaV1.2) in Rat Paraventricular Nucleus
Immuno-Colocalization of TrKA and NGF in Mouse Cerebellar Cortex
Immuno-Colocalization of KCa3.1 and TRPC1 in Mouse Brain
Immuno-Colocalization of Kir4.1 and GFAP in Rat Hippocampal Dentate Gyrus
Immuno-Colocalization of VGLUT2 and Neurokinin Receptor 1 in Rat DRG
Immuno-Colocalization of TRPV1 and NaV1.8 in Rat DRG
Immuno-Colocalization of TRPV1 and mGluR5 in Rat DRG
Immuno-Colocalization of NK1 and mGluR5 in Rat DRG
Immuno-Colocalization of TRPV2 and mGluR5 in Rat DRG
Immuno-Colocalization of Orexin Receptor 1 and Parathyroid Hormone 1 Receptor in Rat Ventromedial Hypothalamus
Immuno-Colocalization of GluR1 and Vesicular GABA Transporter in Human U-87 MG Cells
Immuno-Colocalization of TRPC6 and TRPC3 in Rat Cerebellum
Immuno-Colocalization of P2X7 Receptor and Iba-1 Microglia Marker in Mouse Spinal Cord
Immuno-Colocalization of CaV1.2 and GABA(A) α1 Receptor in Rat Cerebellum