Cat #: STC-690
Alternative Name Mu-Conotoxin BuIIIC
This product is freeze dried. All water molecules have been removed.
Every lot is tried & tested in a relevant biological assay.
Origin Synthetic peptide
MW: 3006 Da.
Purity: >98% (HPLC)
Form Lyophilized powder.
Effective concentration 1 pM - 20 nM.
Modifications Disulfide bonds between Cys5-Cys19, Cys6-Cys25, and Cys15-Cys26. Cys26 - C-terminal amidation.
Molecular formula C116H189N49O34S6.
Activity µ-Conotoxin BuIIIC is a selective NaV1.4 blocker1.
- Holford, M. et al. (2009) Toxicon 53, 90.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to two weeks at 4°C or three months at -20°C.
- Alomone Labs µ-Conotoxin BuIIIC blocks NaV1.4 currents expressed in Xenopus oocytes.A. Time course of µ-Conotoxin BuIIIC (#STC-690) blocking action on NaV1.4 currents. Peak current amplitude was plotted as a function of time. Membrane potential was held at -100 mV and cells were stimulated by a 100 ms voltage step to -10 mV. 20 nM µ-Conotoxin BuIIIC were perfused as indicated by the bar (green) during 5 min. B. Superimposed examples of NaV1.4 channel current in the absence (control) and presence (green) of 20 nM µ-Conotoxin BuIIIC (taken from the experiment in A).
References - Scientific background
- 1. Holford, M. et al. (2009) Toxicon 53, 90.
- μ-conotoxin BuIIIC, is a peptide toxin originally isolated from Conus Bullatus (Bubble cone). It has the characteristic arrangement of six-cysteine residues (-CC-C-C-CC-) and comprises 26 amino acid residues. μ-conotoxin BuIIIC was demonstrated to be a potent irreversible inhibitor of the voltage-gated Na+ channel skeletal muscle subtype NaV1.4, expressed in Xenopus oocytes. 1 μM toxin was shown to block 96% of the channel. The novel structural determinants of BuIIIC along with its ability to potently inhibit NaV1.4 make this conotoxin useful in defining features of the NaV1.4 pharmacophore and thereby facilitate the design of highly subtype-specific ligands that target NaV1.41.
Target NaV1.4 Na+ channels
Net Peptide Content: 100%
Last update: 20/08/2019
µ-Conotoxin BuIIIC (#STC-690) is a highly pure, synthetic, and biologically active peptide toxin.
For research purposes only, not for human use
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