µ-Conotoxin CnIIIC

Mu-Conotoxin CnIIIC
A Blocker of NaV Channels and an Antagonist of nAChRs
    Cat #: STC-640
    Alternative Name Mu-Conotoxin CnIIIC
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 2376 Da.
    Purity: >99% (HPLC)
    Effective concentration 0.5-1 µM.
    Sequence ZGCCNGPKGCSSKWCRDHARCC.
    Modifications Disulfide bonds between Cys3-Cys15, Cys4-Cys21 and Cys10-Cys22. Cys22 – C-terminal amidation. Z-Pyrrolidone carboxylic acid.
      • µ-Conotoxin CnIIIC
    Molecular formula C92H139N35O28S6.
    Activity µ-Conotoxin CnIIIC blocks Nachannels and α3/β2 nAChRs.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • µ-Conotoxin CnIIIC
        Alomone Labs µ-Conotoxin CnIIIC inhibits NaV1.2 currents heterologously expressed in Xenopus oocytes.
        A. Time course of µ-Conotoxin CnIIIC (#STC-640) action on peak current NaV1.2 amplitude. Peak current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage step to -10 mV. 0.5 µM µ-Conotoxin CnIIIC was perfused as indicated by the bar for 5 min. B. Superimposed examples of NaV1.2 channel peak current in the absence (control) and presence (green) of 0.5 µM µ-Conotoxin-CnIIIC (taken from the experiment in A).
    References - Scientific background
    • 1. Favreau, P. et al. (2012) Br. J. Pharmacol166, 1654.
      • µ-Conotoxin CnIIIC, a 22-residue conopeptide from Conus consors belonging to µ-conopeptide family, is a very potent, selective and durable antagonist of NaV channels. This toxin blocks both voltage-gated Na+ channels and neuronal nicotinic acetylcholine receptor (nAChR). µ-Conotoxin CnIIIC inhibits the rat skeletal muscle NaV1.4 with IC50 of 1.3 nM and the rat brain NaV1.2 expressed in HEK-293 cells. A low inhibition is also observed for mouse neuronal NaV1.6 and mouse NaV1.7. µ-Conotoxin CnIIIC modestly blocks nAChR α3/β2 subtype with IC50 values of 450 nM (partially reversible) and, to a lesser extent, α7 and α4/β2 subtypes (reversible) expressed in Xenopus oocytes. in vitro, it decreases twitch tension in mouse hemidiaphragms (IC50 = 150 nM) and displays a high blocking effect in mouse extensor digitorum longus muscles (IC50 = 46 nM).
    Target NaV Na+ channels, α3/β2 nAChR
    Net Peptide Content: 100%
    Last update: 24/09/2019

    µ-Conotoxin CnIIIC (#STC-640) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use