µ-Conotoxin GIIIA

G3.9, Geographutoxin I, GTx-I, Myotoxin I
A Blocker of NaV1.4 Na+ Channel 
Cat #: STC-280
Alternative Name G3.9, Geographutoxin I, GTx-I, Myotoxin I
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide originated from Conus geographus (Geography cone, Nubecula geographus).
    MW: 2609 Da.
    Purity: >99% (HPLC)
    Effective concentration 50-500 nM.
    Sequence RDCCTXXKKCKDRQCKXQRCCA.
    Modifications Disulfide bonds between Cys3-Cys15, Cys4-Cys20 and Cys10-Cys21. X = Hydroxyproline. Ala22 – C-terminal amidation.
    Molecular formula C100H170N38O32S6.
    CAS No.: 129129-65-3.
    Activity µ-Conotoxin GIIIA is a NaV1.4 channel blocker1,2.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • Alomone Labs µ-Conotoxin GIIIA blocks NaV1.4 currents in Xenopus oocytes.
        A. Time course of µ-Conotoxin GIIIA (#STC-280) action on maximum NaV1.4 current amplitude. Maximum peak current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage step to 0 mV. 100 nM µ-Conotoxin-GIIIA was perfused as indicated by the bar (green) for 5 min. B. Superimposed examples of NaV1.4 channel peak current in the absence (control) and presence (green) of 100 nM µ-Conotoxin-GIIIA (taken from the experiment in A).
      • μ-Conotoxins contain 16-25 residues and belongs to the M-superfamily of conopeptides, μ-Conotoxin GIIIA specifically blocks skeletal muscle Na+ channels (NaV1.4) with an IC50 of 1500 nM1.

        NaV channels are targets for many toxins, which bind with high affinity to various sites on the channel protein, µ-Conotoxin selectively binds to the NaV1.4 channel, blocks the pore and disabling its normal function, act as an antagonist for VGSCs1.

        Voltage-gated NaV channels (VGSCs) play a key role in the electric excitability of cells by regulating the influx of sodium ions. VGSCs are responsible for initiating and propagating action potentials, which are essential for the activity of a wide selection of cells and tissues such as neurons, heart and muscle. In mammals, nine different subtypes (NaV1.1-1.9) have been identified, each with distinct distribution.

        VGSCs acts as a potential drug targets for related disorders such as cardiac and neuropathic diseases2,3.

    Target NaV1.4 Na+ channel
    Net Peptide Content: 100%
    Last update: 30/06/2019

    µ-Conotoxin GIIIA (#STC-280) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use