µ-Conotoxin SIIIA

A Blocker of TTX-Sensitive NaV1.2, NaV1.3 and NaV1.4 Voltage-Gated Na+ Channels
Cat #: STC-450
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide originated from Conus striatus (Striated cone).
    MW: 2207 Da.
    Purity: >98% (HPLC)
    Effective concentration 0.5-3 µM.
    Sequence ZNCCNGGCSSKWCRDHARCC.
    Modifications Disulfide bonds between Cys3-Cys13, Cys4-Cys19, Cys8-Cys20. Z1 - Pyrrolidone carboxylic acid, Cys20 - Cysteine amide.
    Molecular formula C83H123N33O27S6.
    Activity µ-Conotoxin SIIIA blocks TTX-sensitive voltage-gated Na+ channels in small-diameter DRG neurons1,2. It also irreversibly blocks NaV1.2, NaV1.3 and NaV1.4 channels expressed in Xenopus oocytes in a dose- and time-dependent manner3. No inhibition is detected on NaV1.5, NaV1.7 or NaV1.8 channels4,5.
      • Bulaj, G. et al. (2005) Biochemistry 44, 7259.

      • Wang, C.Z. et al. (2006) Toxicon 47, 122.

      • Schroeder, C.I. et al. (2008) J. Biol. Chem. 283, 21621.

      • Yao, S. et al. (2008) Biochemistry 47, 10940.

      • Leipold, E. et al. (2011) Neuropharmacology 61, 105.

    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • Alomone Labs µ-Conotoxin SIIIA inhibits NaV1.2 channels heterologously expressed in Xenopus oocytes.
        A. Time course of channel activity and inhibition in the presence of 1 µM  µ-Conotoxin SIIIA (#STC-450) applied for 10 min (indicated as bar). Currents were elicited by application of voltage ramp from a holding potential of -80 mV to 0 mV in 100 msec, delivered every 10 seconds. B. Example of superimposed current traces before (black) and during (green) 1 µM application of µ-Conotoxin SIIIA.
      • Bulaj, G. et al. (2005) Biochemistry 44, 7259.
      • Wang, C.Z. et al. (2006) Toxicon 47, 122.
      • Schroeder, C.I. et al. (2008) J. Biol. Chem. 283, 21621.
      • Yao, S. et al. (2008) Biochemistry 47, 10940.
      • Leipold, E. et al. (2011) Neuropharmacology 61, 105.
      • µ-Conotoxin SIIIA is a synthetic peptide purified to homogeneity, originally isolated from the Conus striatus venom1. µ-Conotoxin SIIIA reversibly blocks TTX-sensitive voltage-gated Na+ channels in small-diameter DRG neurons.1,2 It also irreversibly blocks NaV1.2, NaV1.3 and NaV1.4 channels expressed in Xenopus oocytes in a dose- and time-dependent manner3. No inhibition is detected on NaV1.5, NaV1.7 or NaV1.8 channels4,5.
    Target NaV1.2, NaV1.3, NaV1.4 Na+ channels
    Net Peptide Content: 100%
    Last update: 09/07/2019

    µ-Conotoxin SIIIA (#STC-450) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use