This product is freeze dried. All water molecules have been removed.
Every lot is tried & tested in a relevant biological assay.
Luo, S. et al. (1998) J. Neurosci. 18, 8571.
Alomone Labs α-Conotoxin AuIB inhibits α3/β4 nAChR channels heterologously expressed in Xenopus oocytes.Superimposed traces of α3/β4 nAChR channel current in the absence (black) and presence of different concentrations of α-Conotoxin AuIB (#STC-310). Current amplitudes were plotted as a function of time. Membrane potential was held at -60 mV and oocytes were stimulated by exposure to 20 μM Acetylcholine and 1 μM Atropine, for 1 second every 50 seconds. The indicated different α-Conotoxin AuIB concentrations (inset) were perfused during the period marked in colors.
α-Conotoxin AuIB is a 15 amino acid peptidyl toxin isolated from Conus aulicus1. This toxin inhibits mammalian neuronal α3/β4 nicotinic acetylcholine receptor (nAChR) channels expressed in Xenopus oocytes with an IC50 of 0.75 µM. Furthermore, α-Conotoxin AuIB blocks the α3/β4 receptor with >100-fold higher potency than other receptor subunit combinations1.
In accordance, α-Conotoxin AuIB (1-5 µM) blocks 20-35% of the nicotine-stimulated norepinephrine release from rat hippocampal synaptosomes1 and the nicotinic ACh receptors on dissociated neurons of the rat parasympathetic ganglia with IC50 of 1.2 nM2.
α-Conotoxin AuIB (#STC-310) is a highly pure, synthetic, and biologically active peptide toxin.