α-Conotoxin AuIB

A Potent Antagonist of Mammalian α3/β4 Neuronal nAChR
    Cat #: STC-310
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 1572 Da.
    Purity: >99% (HPLC)
    Effective concentration 0.1-5 μM.
    Sequence GCCSYPPCFATNPDC.
    Modifications Disulfide bonds between Cys2-Cys8 and Cys3-Cys15. Cys15 - C-terminal amidation.
      • α-Conotoxin AuIB
    Molecular formula C65H89N17O21S4.
    CAS No.: 216299-21-7.
    Activity α-Conotoxin AuIB potently and specifically blocks mammalian neuronal nAChR channels α3/β41.
      • Luo, S. et al. (1998) J. Neurosci. 18, 8571.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • α-Conotoxin AuIB
        Alomone Labs α-Conotoxin AuIB inhibits α3/β4 nAChR channels heterologously expressed in Xenopus oocytes.
        Superimposed traces of α3/β4 nAChR channel current in the absence (black) and presence of different concentrations of α-Conotoxin AuIB (#STC-310). Current amplitudes were plotted as a function of time. Membrane potential was held at -60 mV and oocytes were stimulated by exposure to 20 μM Acetylcholine and 1 μM Atropine, for 1 second every 50 seconds. The indicated different α-Conotoxin AuIB concentrations (inset) were perfused during the period marked in colors.
    References - Scientific background
    • 1. Luo, S. et al. (1998) J. Neurosci. 18, 8571.
    • 2. Dutton, J.L. et al. (2002) J. of Biol. Chem. 277, 48849.
      • α-Conotoxin AuIB is a 15 amino acid peptidyl toxin isolated from Conus aulicus1. This toxin inhibits mammalian neuronal α3/β4 nicotinic acetylcholine receptor (nAChR) channels expressed in Xenopus oocytes with an IC50 of 0.75 µM. Furthermore, α-Conotoxin AuIB blocks the α3/β4 receptor with >100-fold higher potency than other receptor subunit combinations1.

        In accordance, α-Conotoxin AuIB (1-5 µM) blocks 20-35% of the nicotine-stimulated norepinephrine release from rat hippocampal synaptosomes1 and the nicotinic ACh receptors on dissociated neurons of the rat parasympathetic ganglia with IC50 of 1.2 nM2.

    Target α3/β4 nAChR
    Net Peptide Content: 100%
    Last update: 20/08/2019

    α-Conotoxin AuIB (#STC-310) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use