α-Conotoxin EIIB

Alpha-conotoxin EIIB
A Potent Antagonist of α1/β1/δ/γ nAChR
    Cat #: STC-230
    Alternative Name Alpha-conotoxin EIIB
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 1754.5 Da.
    Purity: >99% (HPLC)
    Form Lyophilized powder.
    Effective concentration 1-50 nM.
    Sequence QTXGCCWHPACGKNRC.
    Modifications Disulfide bonds between Cys5-11, Cys6-16. Cys16 – C-terminal amidation. Hydroxyprolines at Pro3 and Pyrrolidone carboxylic acid at Q1.
      • α-Conotoxin EIIB
    Molecular formula C71H105N25O20S4.
    Activity α-Conotoxin EIIB is a potent α1/β1/δ/γ nAChR antagonist1.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • α-Conotoxin EIIB
        Alomone Labs α-Conotoxin EIIB inhibits muscle nicotinic ACh receptors expressed in Xenopus oocytes.
        A. Representative time course of α-Conotoxin EIIB (#STC-230) inhibition of α1/β1/γ/δ nAChR. Currents were elicited every 50 sec by transient application of 20 µM ACh, while membrane potential was held at -80 mV, and inhibited by 50 nM α-Conotoxin-EIIB (horizontal bar). B. Superimposed traces of α1/β1/γ/δ nAChR currents upon application of control and of 50 nM α-Conotoxin-EIIB (taken from the recording in A).
    References - Scientific background
      • α-Conotoxin EIIB is a peptide toxin originally isolated from Conus ermineus (Atlantic fish-hunting cone) venom. It is a potent α1/β1/δ/γ nicotinic acetylcholine receptor antagonist and displays a Ki of 2.2 ± 0.7 nM1. The peptide toxin is stabilized by six hydrogen bonds and disulfides between residues 2 and 7 and 3 and 132.

        Nicotinic acetylcholine receptors (nAChRs) are ionotropic multisubunit, neurotransmitter-gated receptors of the cholinergic system. They are assembled from one or more α subunits (α1-α10) alone or together with one or more β subunits (β1–β4)3. Other subunits also co-assemble in the formation of the receptor channel.

    Target Muscle nAChR
    Net Peptide Content: 100%
    Last update: 20/08/2019

    α-Conotoxin EIIB (#STC-230) is a highly pure, synthetic and biologically active peptide toxin.

    For research purposes only, not for human use