α-Conotoxin GI

α-Conotoxin G1
A Potent Antagonist of α1/β1/δ/γ nAChR
    Cat #: STC-500
    Alternative Name α-Conotoxin G1
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 1438 Da.
    Purity: >98% (HPLC)
    Effective concentration 10 nM - 1 μM.
    Sequence ECCNPACGRHYSC.
    Modifications Disulfide bonds between Cys2-Cys7, Cys3-Cys13. Cys13 - Cysteine amide.
      • α-Conotoxin GI
    Molecular formula C55H80N20O18S4.
    CAS No.: 76862-65-2.
    Activity α-Conotoxin GI inhibits α/δ site on mouse muscle-derived BC3H-1 nicotinic acetylcholine receptors (nAChR) expressed on postsynaptic membranes. Other site (α/γ site) on nicotinic receptors from Torpedo californica electric organ is also inhibited by α-Conotoxin GI1,2.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • α-Conotoxin GI
        Alomone Labs α-Conotoxin GI inhibits the muscle (α1/β1/δ/γ) nAChR channels heterologously expressed in Xenopus oocytes.
        A. Time course of α-Conotoxin GI (#STC-500) action on α1/β1/δ/γ nAChR channels. Current amplitudes were plotted as a function of time. Membrane potential was held at –60 mV and oocytes were stimulated by exposure to 1 mM acetylcholine every 100 seconds. 100 nM α-Conotoxin GI were perfused during the period marked by the bar. B. Superimposed traces of α1/β1/δ/γ nAChR channel current in the absence and presence of 100 nM α-Conotoxin GI (taken from the experiment in A).
    References - Scientific background
      • α-Conotoxin GI is a 13 amino acid peptidyl toxin isolated from the Conus geographus (Geography cone) venom1,2,3. It belongs to the Conotoxin A superfamily and reversibly blocks α/δ nicotinic ACh channel receptors (nAChR).

        α-Conotoxin GI reversibly inhibits the high affinity α/δ site on mouse muscle-derived BC3H-1 receptor, a nAChR expressed on postsynaptic membranes4,5. Other low site (α/γ site) on nicotinic receptors from Torpedo californica electric organ is also inhibited by α-Conotoxin GI5.

    Target α1/β1/δ/γ nAChR
    Net Peptide Content: 100%
    Last update: 20/08/2019

    α-Conotoxin GI (#STC-500) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use