α-Conotoxin ImI-ATTO-590

A Potent Antagonist of Mammalian α3/β2 nAChR Conjugated to the Fluorescent Dye ATTO-590
  • New
Cat #: C-290-AR
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic
    Source Modified synthetic peptide
    MW: 1924 Da.
    Purity: >95%
    Formulation Lyophilized powder.
    Effective concentration 1 – 2 µM.
    Sequence GCCSDPRCAWRC.
    Modifications Disulfide bonds between Cys2-Cys8, Cys3-Cys12. Cys12 - C-terminal amidation.
    Label ATTO-590. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO-590 maximum absorption is 593 nm and maximum fluorescence is at 622 nm. The fluorescence is excited most efficiently in the 575-610 nm range. The extent of labeling is 1 molecule of dye per molecule α-Conotoxin ImI.
    Activity α-Conotoxin ImI potently and specifically blocks mammalian neuronal nAChR (α3/β2 > α7 > α3/β4)1.
    References - Activity
    1. McIntosh, J.M. et al. (1994) J. Biol. Chem. 269, 16733.
    Storage before reconstitution Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Reconstitution Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min). Avoid exposure to light.
    Storage after reconstitution Up to two weeks at 4°C or three months at -20°C. Avoid exposure to light.
    Our bioassay
    Alomone Labs α-Conotoxin ImI-ATTO-590 inhibits α3/β2 nAChRs expressed in Xenopus oocytes.
    A. Representative time course of α3/β2 current inhibition by 1.5 µM α-Conotoxin ImI-ATTO-590 (#C-290-AR). Currents were elicited every 50 sec by transient applications of 20 µM ACh, while membrane potential was held at -80 mV, and inhibited by 1.5 µM labeled toxin (green). B. Superimposed traces of α3/β2 currents evoked by ACh (arrow) after application of control (black) and of 1.5 µM α-Conotoxin ImI-ATTO-590 (green). Taken from the recording in A.
    Alomone Labs α-Conotoxin ImI-ATTO-590 stains rat cerebellum.
    Rat cerebellum sections were incubated with 25 nM α-Conotoxin ImI-ATTO-590 (#C-290-AR). A. DAPI (blue) shows distribution outlines in the layers of the cerebellum (arrow). B. α-Conotoxin ImI-ATTO-590 stains (red) neuronal clusters (arrow) in the granule layer (G). There was weak nonspecific labeling of the molecular layer (MOL) and nearly no labeling in the white matter (WM) layer.
    Alomone Labs α-Conotoxin ImI-ATTO-590 stains rat deep cerebellar nuclei.
    Rat deep cerebellar nuclei sections were incubated with 25 nM α-Conotoxin ImI-ATTO-590 (#C-290-AR). A. DAPI shows distribution outlines in the layers of the cerebellum. B. α-Conotoxin ImI-ATTO-590 stains cerebellar nuclei neurons (arrows).
    References - Scientific Background
    1. McIntosh, J.M. et al. (1994) J. Biol. Chem. 269, 16733.
    2. Johnson, D.S. et al. (1995) Mol. Pharmacol. 48, 194.
    Scientific background α-Conotoxin ImI is a 12 amino acid peptidyl toxin isolated from Conus Imperialis venom1, which blocks mammalian neuronal nAChRs located on postsynaptic membranes. This toxin inhibits mammalian neuronal nAChRs (α3/β2 > α7 > α3/β4) in a voltage-independent manner and has no effect on nAChRs composed of α2/β2, α3/β2, α4/β2, α2/β4, α3/β4, or α4/β4 subunits1,2.
    Target α3/β2 nAChR
    Net Peptide Content: 100%

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    Last update: 25/11/2018

    α-Conotoxin ImI-ATTO-590 (#C-290-AR) is a highly pure, synthetic, and biologically active conjugated peptide toxin.

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    For research purposes only, not for human use