This product is freeze dried. All water molecules have been removed.
Every lot is tried & tested in a relevant biological assay.
- McIntosh, J.M. et al. (1994) J. Biol. Chem. 269, 16733.
Alomone Labs α-Conotoxin ImI-ATTO-590 inhibits α3/β2 nAChRs expressed in Xenopus oocytes.A. Representative time course of α3/β2 current inhibition by 1.5 µM α-Conotoxin ImI-ATTO-590 (#C-290-AR). Currents were elicited every 50 sec by transient applications of 20 µM ACh, while membrane potential was held at -80 mV, and inhibited by 1.5 µM labeled toxin (green). B. Superimposed traces of α3/β2 currents evoked by ACh (arrow) after application of control (black) and of 1.5 µM α-Conotoxin ImI-ATTO-590 (green). Taken from the recording in A.Alomone Labs α-Conotoxin ImI-ATTO-590 stains rat cerebellum.Rat cerebellum sections were incubated with 25 nM α-Conotoxin ImI-ATTO-590 (#C-290-AR). A. DAPI (blue) shows distribution outlines in the layers of the cerebellum (arrow). B. α-Conotoxin ImI-ATTO-590 stains (red) neuronal clusters (arrow) in the granule layer (G). There was weak nonspecific labeling of the molecular layer (MOL) and nearly no labeling in the white matter (WM) layer.Alomone Labs α-Conotoxin ImI-ATTO-590 stains rat deep cerebellar nuclei.Rat deep cerebellar nuclei sections were incubated with 25 nM α-Conotoxin ImI-ATTO-590 (#C-290-AR). A. DAPI shows distribution outlines in the layers of the cerebellum. B. α-Conotoxin ImI-ATTO-590 stains cerebellar nuclei neurons (arrows).
α-Conotoxin ImI is a 12 amino acid peptidyl toxin isolated from Conus Imperialis venom1, which blocks mammalian neuronal nAChRs located on postsynaptic membranes. This toxin inhibits mammalian neuronal nAChRs (α3/β2 > α7 > α3/β4) in a voltage-independent manner and has no effect on nAChRs composed of α2/β2, α3/β2, α4/β2, α2/β4, α3/β4, or α4/β4 subunits1,2.
α-Conotoxin ImI-ATTO-590 (#C-290-AR) is a highly pure, synthetic, and biologically active conjugated peptide toxin.
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