α-Conotoxin PeIA

An Antagonist of nAChR and N-Type Ca2+ Channels
    Cat #: STC-970
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 1652 Da.
    Purity: >98% (HPLC)
    Effective concentration 20-50 nM.
    Sequence GCCSHPACSVNHPELC.
    Modifications Disulfide bonds between Cys2-Cys8 and Cys3-Cys16. Cys16 – C-terminal amidation.
      • α-Conotoxin PeIA
    Molecular formula C65H98N22O21S4.
    Activity α-Conotoxin PeIA blocks α9/α10 and α3/β2 nAChR1. Also inhibits N-type Ca2+ channels2.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two week at 4°C or three months at -20°C.
      • α-Conotoxin PeIA
        Alomone Labs α-Conotoxin PeIA inhibits α3/β2 nAChR heterologously expressed in Xenopus oocytes.
        A. Time course of α-Conotoxin PeIA (#STC-970) action on α3/β2 nAChR. Current amplitude were plotted as a function of time. Membrane potential was held at -80 mV and oocytes were stimulated by exposing them to 100 µM acetylcholine (Ach) every 100 sec. 50 nM (for 300 sec), 100 nM (for 300 sec) and 200 nM (for 200 sec) α-Conotoxin PeIA were perfused during the period marked by the bar. B. Superimposed traces of α3/β2 nAChR channel current in the absence (black) and presence of different concentrations of α-Conotoxin PeIA (taken from experiment in A).
    References - Scientific background
    • 1. McIntosh, J.M. et al. (2005) J. Biol. Chem. 280, 30107.
    • 2. Daly, N.L. et al. (2011) J. Biol. Chem. 286, 10233.
      • α-Conotoxin PeIA is a peptide toxin originally isolated from the Conus pergrandis (Grand cone) venom1. This toxin belongs to the α-Conotoxin family which acts on the postsynaptic membrane by binding and inhibiting nicotinic acetylcholine receptors (nAChR)1.

        It potently and reversibly blocks α9/α10 nAChR (IC50 = 6.9 nM) and α3/β2 (IC50 = 23 nM) and discriminates between rat α9/α10 and α7 nACh recetors1. It also inhibits N-type Ca2+ channels (CaV2.2) (IC50 = 1.1 nM) via the activation of the GABA(B) G-protein coupled receptor in DRG neurons2.

    Target nAChR, CaV2.2 Ca2+ channels
    Net Peptide Content: 100%
    Last update: 24/09/2019

    α-Conotoxin PeIA (#STC-970) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use