Certificate of Analysis

α1-Adrenergic Receptor Antibody Explorer Kit

A Screening Package of α1-Adrenergic Receptor Antibodies Economically Priced
Cat #: AK-490 6 Vials

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-α1A-Adrenergic Receptor (extracellular) Antibody Cat #: AAR-015

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, R
Immunogen
Peptide EDETI*SQINEEPG(C), corresponding to amino acid residues 171-183 of human α1A-adrenoceptor with replacement of cysteine 176 (C176) with serine (*S) (Accession P35348). 2nd extracellular loop.
Homology
Human, rat, mouse, bovine - 12/13 amino acid residues identical.

Anti-α1B-Adrenergic Receptor (extracellular) Antibody Cat #: AAR-018

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, R
Immunogen
Peptide (C)KNANFTGPNQTSSNS, corresponding to amino acid residues 21-35 of human α1B-adrenoceptor (Accession P35368). Extracellular, N-terminus.
Homology
Mouse - 14/15 amino acid residues identical; rat - 13/15 amino acid residues identical.

Anti-α1D-Adrenergic Receptor (extracellular) Antibody Cat #: AAR-019

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)EPVPPDERF*SGITEE, corresponding to amino acid residues 231-245 of rat α1D-adrenoceptor with replacement of cysteine 240 (C240) with serine (*S) (Accession P23944). 3rd extracellular loop.
Homology
Rat, human, mouse - 14/15 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.