Overview
Cat #: BLP-AR015
Type: Synthetic peptide
Form: Lyophilized powder
α1A-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR015) is the original antigen used for immunization during Anti-α1A-Adrenergic Receptor (extracellular) Antibody (#AAR-015) generation. The blocking peptide binds and ‘blocks’ Anti-α1A-Adrenergic Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of Human PC3 prostate carcinoma cell line lysate (lanes 1 and 3) and rat brain membrane (lanes 2 and 4):1,2. Anti-α1A-Adrenergic Receptor (extracellular) Antibody (#AAR-015), (1:200).
3,4. Anti-α1A-Adrenergic Receptor (extracellular) Antibody, preincubated with α1A-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR015). - Expression of α1A-Adrenergic receptor in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-α1A-Adrenergic Receptor (extracellular) Antibody (#AAR-015), (1:100). A. α1A-adrenoceptor (green) appears in fibers of Bergmann glia (triangle points at an example). B. S100β (red), a marker of Bergmann glia, is stained in the same section. C. Merge of the images demonstrates expression of α1A-adrenoceptor in fibers of Bergmann glia. α1A-adrenoceptor is also expressed in cells in the molecular layer that are s100β negative (arrow points at an example). DAPI is used as the counterstain (blue).
Properties
Sequence
Accession (Uniprot) Number P35348
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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