Overview
Type: Synthetic peptide
Form: Lyophilized powder
α1A-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR015) is the original antigen used for immunization during Anti-α1A-Adrenergic Receptor (extracellular) Antibody (#AAR-015) generation. The blocking peptide binds and ‘blocks’ Anti-α1A-Adrenergic Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of Human PC3 prostate carcinoma cell line lysate (lanes 1 and 3) and rat brain membrane (lanes 2 and 4):1,2. Anti-α1A-Adrenergic Receptor (extracellular) Antibody (#AAR-015), (1:200).
3,4. Anti-α1A-Adrenergic Receptor (extracellular) Antibody, preincubated with α1A-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR015).
Expression of α1A-Adrenergic receptor in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-α1A-Adrenergic Receptor (extracellular) Antibody (#AAR-015), (1:100). A. α1A-adrenoceptor (green) appears in fibers of Bergmann glia (triangle points at an example). B. S100β (red), a marker of Bergmann glia, is stained in the same section. C. Merge of the images demonstrates expression of α1A-adrenoceptor in fibers of Bergmann glia. α1A-adrenoceptor is also expressed in cells in the molecular layer that are s100β negative (arrow points at an example). DAPI is used as the counterstain (blue).
Properties
Sequence
- EDETI*SQINEEPG(C), corresponding to amino acid residues 171-183 of human α1A-adrenoceptor with replacement of cysteine 176 (C176) with serine (*S) (Accession P35348).
Accession (Uniprot) Number P35348
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
