Overview
Type: Synthetic peptide
Form: Lyophilized powder
α1B-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR018) is the original antigen used for immunization during Anti-α1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018) generation. The blocking peptide binds and ‘blocks’ Anti-α1B-Adrenergic Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of α1B-Adrenoceptor in rat brain (lanes 1 and 3), rat kidney (lanes 2 and 4) and GH3 cell line (lanes 5 and 6) lysates:1,2,5. Anti-α1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018), (1:200).
3,4,6. Anti-α1B-Adrenergic Receptor (extracellular) Antibody, preincubated with α1B-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR018).
Expression of α1B-Adrenoceptor in rat cerebellumImmunohistochemical staining of frozen section of rat cerebellum using Anti-α1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018), (1:100). α1B-Adrenoceptor (red) is detected in Purkinje cells. Hoechst 33342 was used as the counterstain.
Properties
Sequence
- (C)KNANFTGPNQTSSNS, corresponding to amino acid residues 21-35 of human α1B-adrenoceptor (Accession P35368).
Accession (Uniprot) Number P35368
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
