Cat #: BLP-AR018
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-α1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018) and Anti-α1B-Adrenergic Receptor (extracellular)-ATTO Fluor-488 Antibody (#AAR-018-AG). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of α1B-Adrenoceptor in rat brain (lanes 1 and 3), rat kidney (lanes 2 and 4) and GH3 cell line (lanes 5 and 6) lysates:1,2,5. Anti-α1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018), (1:200).
3,4,6. Anti-α1B-Adrenergic Receptor (extracellular) Antibody, preincubated with α1B-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR018).
- Expression of α1B-Adrenoceptor in rat cerebellumImmunohistochemical staining of frozen section of rat cerebellum using Anti-α1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018), (1:100). α1B-Adrenoceptor (red) is detected in Purkinje cells. Hoechst 33342 was used as the counterstain.
- (C)KNANFTGPNQTSSNS, corresponding to amino acid residues 21-35 of human α1B-adrenoceptor (Accession P35368).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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