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Certificate of Analysis

α2-Adrenergic Receptor Antibody Explorer Kit

A Screening Package of α2-Adrenergic Receptor Antibodies Economically Priced

Cat #: AK-495
Size: 3 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-α2A-Adrenergic Receptor (extracellular) Antibody Cat #: AAR-020

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, WB Reactivity: M, R
Immunogen
Peptide (C)DAGNSSWNGTEAPG, corresponding to amino acid residues 7-20 of rat α2A-adrenoceptor (Accession P22909). Extracellular, N-terminus.
Homology
Mouse - identical; human - 13/14 amino acid residues identical.

Anti-α2B-Adrenergic Receptor (extracellular) Antibody Cat #: AAR-021

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: R
Immunogen
Peptide (C)RPEPRGLPQ*SELNQE, corresponding to amino acid residues 160-174 of rat α2B-adrenoceptor with replacement of cysteine 169 (C169) with serine (*S) (Accession P19328). 2nd extracellular loop.
Homology
Rat - 14/15 amino acid residues identical; mouse - 13/15 amino acid residues identical; human - 10/14 amino acid residues identical.

Anti-α2C-Adrenergic Receptor (extracellular) Antibody Cat #: AAR-022

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: R
Immunogen
Peptide (C)RRPDGAAYPQ*SGLNDE, corresponding to amino acid residues 192-207 of rat α2C-adrenoceptor with replacement of cysteine 202 (C202) with serine (*S) (Accession P22086). 2nd extracellular loop.
Homology
Rat, mouse - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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