Certificate of Analysis

β-Adrenergic Receptor Antibody Explorer Kit

A Screening Package of β-Adrenergic Receptor Antibodies Economically Priced
Cat #: AK-500 6 Vials

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-β1-Adrenergic Receptor (extracellular) Antibody Cat #: AAR-023

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)RAESDEARR(S)YNDP, corresponding to amino acid residues 200-214 of rat ADRB1 (Accession P18090). 2nd extracellular loop.
Homology
Rat, Mouse, human - 14/15 amino acid residues identical.

Anti-β2-Adrenergic Receptor (extracellular) Antibody Cat #: AAR-016

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)NGSRAPDHDVTQERDE, corresponding to amino acid residues 15-30 of mouse ADRB2 (Accession P18762). Extracellular, N-terminus.
Homology
Human - 15/16 amino acid residues identical; rat - 14/16 amino acid residues identical.

Anti-β3-Adrenergic Receptor (extracellular) Antibody Cat #: AAR-017

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide DAEAQESH*SNPRC, corresponding to amino acid residues 180-192 of mouse ADRB3 with replacement of cysteine 186 (C186) with serine (*S) (Accession P25962). 2nd extracellular loop.
Homology
Rat - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.