κ-Conotoxin RIIIJ

κ-M-RIIIJ
Voltage-Gated K+ Channel Blocker
Cat #: STC-660
Alternative Name κ-M-RIIIJ
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Conus radiatus (Rayed Cone).
    Source Synthetic peptide
    MW: 2807.9 Da.
    Purity: >98% (HPLC)
    Effective concentration 30 nM.
    Sequence LXXCCTXXKKHCXAXACKYKXCCKS.
    Modifications Disulfide bonds between Cys4-Cys17, Cys5-Cys22 and Cys12-Cys23. X = Hydroxyproline.
    Molecular formula C117H184N32O36S6.
    Activity κ-Conotoxin RIIIJ is a blocker of KV1.2 channels1.
      • Chen, P. et al. (2010) J. Biol. Chem285, 14882.

    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • Alomone Labs κ-Conotoxin RIIIJ inhibits KV1.2 currents in Xenopus oocytes.
        A. Time course of κ-Conotoxin-RIIIJ (#STC-660) action on Kv1.2 currents. Current amplitudes were plotted as a function of time. Membrane potential was held at –80 mV and oocytes were stimulated by a 100 ms voltage ramp to 0 mV. 50 nM κ-Conotoxin-RIIIJ were perfused as indicated by the bar (green) at -15 mV for 160 sec. B. Superimposed examples of KV1.2 channel current in the absence (control) and presence of 50 nM κ-Conotoxin-RIIIJ (taken from the experiment in A).
      • Hille, B. (1992) Ionic Channels of Excitable Membranes, 2nd Ed. Sunderland, MA: Sinauer Associates.
      • Gulbis J. M. et al. (2002) Novartis Found. Symp245, 127
      • Chen, P. et al. (2010) J. Biol. Chem285, 14882.
      • A number of conotoxins isolated from Conus venoms interact with K+ channels.

        κ-conotoxin RIIIJ (κ-M-RIIIJ), is a 25 amino-acid peptidyl toxin belonging to the conotoxin M superfamily. This conopeptide inhibits the homotetrameric human KV1.2 channels (IC50 = 33 nM)3. κ-M-RIIIJ was found to exert cardioprotective effects, presumably due to the high affinity of this peptide for mammalian KV1.2 and KV1.2- containing heteromultimers3.

        Voltage-gated K+ channels represent a structurally and functionally diverse group of membrane proteins. These channels establish the resting membrane potential and modulate the frequency and duration of action potentials in nerve and muscle1,2.

    Target KV1.2 K+ channels
    Net Peptide Content: 100%
    Last update: 14/03/2019

    κ-Conotoxin RIIIJ (#STC-660) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use