Bioassay Tested
Every lot is tried & tested in a relevant biological assay.
Our Bioassay
- Chen, P. et al. (2010) J. Biol. Chem. 285, 14882.
Alomone Labs κ-Conotoxin RIIIJ inhibits KV1.2 currents in Xenopus oocytes.A. Time course of κ-Conotoxin-RIIIJ (#STC-660) action on Kv1.2 currents. Current amplitudes were plotted as a function of time. Membrane potential was held at –80 mV and oocytes were stimulated by a 100 ms voltage ramp to 0 mV. 50 nM κ-Conotoxin-RIIIJ were perfused as indicated by the bar (green) at -15 mV for 160 sec. B. Superimposed examples of KV1.2 channel current in the absence (control) and presence of 50 nM κ-Conotoxin-RIIIJ (taken from the experiment in A).
- Hille, B. (1992) Ionic Channels of Excitable Membranes, 2nd Ed. Sunderland, MA: Sinauer Associates.
- Gulbis J. M. et al. (2002) Novartis Found. Symp. 245, 127
- Chen, P. et al. (2010) J. Biol. Chem. 285, 14882.
κ-conotoxin RIIIJ (κ-M-RIIIJ), is a peptide toxin belonging to the conotoxin M superfamily, and was originally isolated from Conus radiatus (Rayed cone) venom. This conopeptide inhibits the homotetrameric human KV1.2 channels (IC50 = 33 nM)3. κ-M-RIIIJ was found to exert cardioprotective effects, presumably due to the high affinity of this peptide for mammalian KV1.2 and KV1.2- containing heteromultimers3.
Voltage-gated K+ channels represent a structurally and functionally diverse group of membrane proteins. These channels establish the resting membrane potential and modulate the frequency and duration of action potentials in nerve and muscle1,2.
κ-Conotoxin RIIIJ (#STC-660) is a highly pure, synthetic, and biologically active peptide toxin.
Applications
Specifications
Scientific Background
Citations
- Smith, K.E. et al. (2015) J. Neurosci. 35, 11221.
