This product is freeze dried. All water molecules have been removed.
Every lot is tried & tested in a relevant biological assay.
Teramoto, T. et al. (1993) Biochem. Biophys. Res. Commun. 196, 134.
Alomone Labs ω-Agatoxin TK inhibits CaV2.1 channels heterologously expressed in Xenopus oocytes.A. Time course of ω-Agatoxin TK (#STA-530) action on CaV2.1 currents. Maximum current amplitude was plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage ramp to +50 mV in the presence of 2 mM Ba2+. 1 µM ω-Agatoxin TK was perfused as indicated by the bar for 300 s (green). B. Superimposed example of CaV2.1 channel current in the absence (control) and presence (green) of 1 µM ω-Agatoxin TK (taken from the experiment in A).Alomone Labs ω-Agatoxin TK inhibits P-type CaV2.1 channel currents heterologously expressed in HEK 293T cells.Cells were transiently transfected with the α1A, β3 and α2δ subunits. The currents were elicited by 40 ms voltage ramp from holding potential of -100 mV to +60 mV, applied every 10 sec using whole-cell voltage clamp technique. A. Superimposed traces of CaV2.1 currents under control conditions (black) and following 5 min perfusion with 200 nM ω-Agatoxin TK (green). B. Time course of CaV2.1 peak current amplitude change as a result of 200 nM toxin application (ω-Agatoxin TK perfusion is indicated by the horizontal bar).
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ω-Agatoxin TK is a peptide toxin originally isolated from Agelenopsis aperta spider venom. ω-Agatoxin TK was shown to be a selective and reversible blocker of CaV2.1 (P/Q type) channels1. Originally the toxin was designated ω-Agatoxin IVB5-8.
ω-Agatoxin TK (#STA-530) is a highly pure, synthetic, and biologically active peptide toxin.
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