ω-Conotoxin CnVIIA

A Specific Blocker of N-Type Ca2+ Channels 
    Cat #: STC-800
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 2848 Da.
    Purity: >98% (HPLC)
    Effective concentration 1-5 µM.
    Sequence CKGKGAXCTRLMYDCCHGSCSSSKGRC.
    Modifications Disulfide bonds between Cys1-Cys16, Cys8-Cys20 and Cys15-Cys27. X = Hydroxyproline. Cys27 - C-terminal amidation.
      • ω-Conotoxin CnVIIA
    Molecular formula C110H179N39O36S7.
    Activity ω-Conotoxin CNVIIA inhibits N-type Ca2+ channels1.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • ω-Conotoxin CnVIIA
        Alomone Labs ω-Conotoxin CnVIIA inhibits CaV2.2 channels heterologously expressed in Xenopus oocytes.
        A. Time course of ω-Conotoxin CnVIIA (#STC-800) action on CaV2.2 + α2δ1 + β1 currents. Peak current amplitude was plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage ramp to +50 mV. 1 µM ω-Conotoxin CnVIIA (applied for 180 sec, green) was perfused during the period marked by the bar, as indicated and showed 75% inhibition of control current. B. Superimposed traces of channel current in the absence (black) and presence (green) of 1 µM ω-Conotoxin CnVIIA (taken from experiment in A).
    References - Scientific background
    • 1. Qin, N. et al. (2002) Mol. Pharmacol62, 485.
    • 2. Favreau, P. et al. (2001) Biochemistry 40, 14567.
    • 3. Lewis R.J. et al. (2012) Pharmacol. Rev. 64, 259.
      • Ca2+ channels have been classified pharmacologically and biophysically. The channels are designated L-, T-, N-, P-, Q- and R-type. Each channel has different voltage ranges and rates for activation and inactivation. The N-type channels are typically inactivated more rapidly than the L-, P- and Q-types1.

        ω-Conotoxin CNVIIA is a specific voltage-sensitive calcium channel blocker. It was first isolated from the fish-hunting cone snail Conus consors venom. This peptide consists of 27 amino acid residues folded by 3 disulfide bridges2. ω-Conotoxin CNVIIA exhibits a clear selectivity for N-type specific voltage-sensitive Ca2+ channels2. The therapeutic potential of ω-conotoxins is based on their ability to selectively inhibit mammalian CaV isoforms expressed in ascending pain pathways (especially CaV2.2, which underlies the N-type current present in sensory neurons) and to produce antinociception in animal models of pain3.

    Target N-type Ca2+ channels
    Net Peptide Content: 100%
    Last update: 20/08/2019

    ω-Conotoxin CnVIIA (#STC-800) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use