ω-Conotoxin CnVIIA

A Specific Blocker of N-Type Ca2+ Channels 
    Cat #: STC-800
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 2848 Da.
    Purity: >98% (HPLC)
    Effective concentration 1-5 µM.
    Sequence CKGKGAXCTRLMYDCCHGSCSSSKGRC.
    Modifications Disulfide bonds between Cys1-Cys16, Cys8-Cys20 and Cys15-Cys27. X = Hydroxyproline. Cys27 - C-terminal amidation.
    Structure
    • ω-Conotoxin CnVIIA
    Molecular formula C110H179N39O36S7.
    Activity ω-Conotoxin CNVIIA inhibits N-type Ca2+ channels1.
    References-Activity
    1. Favreau, P. et al. (2001) Biochemistry 40, 14567.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • ω-Conotoxin CnVIIA
      Alomone Labs ω-Conotoxin CnVIIA inhibits CaV2.2 channels heterologously expressed in Xenopus oocytes.
      A. Time course of ω-Conotoxin CnVIIA (#STC-800) action on CaV2.2 + α2δ1 + β1 currents. Peak current amplitude was plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage ramp to +50 mV. 1 µM ω-Conotoxin CnVIIA (applied for 180 sec, green) was perfused during the period marked by the bar, as indicated and showed 75% inhibition of control current. B. Superimposed traces of channel current in the absence (black) and presence (green) of 1 µM ω-Conotoxin CnVIIA (taken from experiment in A).
    References - Scientific background
    1. Qin, N. et al. (2002) Mol. Pharmacol62, 485.
    2. Favreau, P. et al. (2001) Biochemistry 40, 14567.
    3. Lewis R.J. et al. (2012) Pharmacol. Rev. 64, 259.
    Scientific background

    Ca2+ channels have been classified pharmacologically and biophysically. The channels are designated L-, T-, N-, P-, Q- and R-type. Each channel has different voltage ranges and rates for activation and inactivation. The N-type channels are typically inactivated more rapidly than the L-, P- and Q-types1.

    ω-Conotoxin CNVIIA is a specific voltage-sensitive calcium channel blocker. It was first isolated from the fish-hunting cone snail Conus consors venom. This peptide consists of 27 amino acid residues folded by 3 disulfide bridges2. ω-Conotoxin CNVIIA exhibits a clear selectivity for N-type specific voltage-sensitive Ca2+ channels2. The therapeutic potential of ω-conotoxins is based on their ability to selectively inhibit mammalian CaV isoforms expressed in ascending pain pathways (especially CaV2.2, which underlies the N-type current present in sensory neurons) and to produce antinociception in animal models of pain3.

    Target N-type Ca2+ channels
    Net Peptide Content: 100%
    Last update: 24/01/2020

    ω-Conotoxin CnVIIA (#STC-800) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Shipping and Ordering information