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ω-Conotoxin CVIA

A Blocker of N-Type CaV Channels 

Cat #: STC-750
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 2642 Da
    Purity: >99% (HPLC)
    Effective concentration 50-200 nM.
    Sequence CKSTGASCRRTSYDCCTGSCRSGRC.
    Modifications Disulfide bonds between Cys1-Cys16, Cys8-Cys20 and Cys15-Cys25.  C25 - C-terminal amidation.
    Structure
    Molecular formula C97H161N39O36S6.
    Activity ω-Conotoxin CVIA is an N-type Ca2+ channel blocker1.
    References-Activity
    1. Lewis, R.J. et al. (2000) J. Biol. Chem. 275, 35335.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs ω-Conotoxin CVIA inhibits CaV2.2 channels expressed in Xenopus oocytes.
      Alomone Labs ω-Conotoxin CVIA inhibits CaV2.2 channels expressed in Xenopus oocytes.
      A. Time course of ω-Conotoxin CVIA (#STC-750) action on maximum CaV2.2 channels (α1B + α2δ1 + β1) current elicited in 2 mM Ba2+. Peak current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage step to 50 mV. 50 nM ω-Conotoxin CVIA was perfused as indicated by the bar (green) for 5 min. B. Superimposed examples of CaV2.2 channel maximum peak current in the absence (control) and presence (green) of 50 nM ω-Conotoxin CVIA (taken from the experiment in A).
    References - Scientific background
    1. Catterall, W.A. et al. (2005) Pharmacol. Rev. 57, 411.
    2. Azimi-Zonooz, A. and Litzinger, M.J. (1992) Int. J. Dev. Neurosci. 10, 447.
    3. Lewis, R.J. et al. (2000) J. Biol. Chem. 275, 35335.
    Scientific background

    CaV channels mediate Ca2+ influx through cell membrane as a response to membrane depolarization. N-type channels require strong depolarization for activation and are mostly expressed in neurons where they initiate neurotransmission in fast synapses and mediate Ca2+ entry into cell bodies and dendrites1.

    ω-conotoxin CVIA is a 27 amino acid neuropeptide2 belonging to a class of four new ω-conotoxins (CVIA-D) extracted from the venom of the Conus Catus snail. These peptides inhibit neuronal voltage sensitive Ca2+ channels in mammals. Interestingly, unlike other ω-conotoxins CVID has a loop 4 sequence and has the highest affinity to N-type channels over other types of Ca2+ channels while CVIA shows a lower affinity to N-type channels.

    CVIA is efficient in the management of severe pain, has clinical potential in ischemic brain injury and was also found to inhibit the contraction of electrically stimulated rat vas deferens with an IC50 of 205 nM3.

    Target CaV2.2 channels
    Peptide Content: 100%
    Last update: 06/11/2022

    ω-Conotoxin CVIA (#STC-750) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use
    Shipping and Ordering information