ω-Conotoxin CVIB

A Blocker of N-, P/Q-Type Ca2+ Channels
    Cat #: STC-410
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 2710 Da.
    Purity: >98% (HPLC)
    Effective concentration 1-10 nM.
    Sequence CKGKGASCRKTMYDCCRGSCRSGRC.
    Modifications Disulfide bonds between Cys1-Cys16, Cys8-Cys20 and Cys15-Cys25. Cys25 - C-terminal amidation.
      • ω-Conotoxin CVIB
    Molecular formula C102H173N41O32S7.
    Activity ω-Conotoxin CVIB is a N-, P- and Q-type calcium channel blocker1.
      • Lewis, R.J. et al. (2000) J. Biol. Chem. 275, 35335.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • ω-Conotoxin CVIB
        Alomone Labs ω-Conotoxin CVIB blocks CaV2.2 currents heterologously expressed in Xenopus oocytes.
        A. Time course of ω-Conotoxin CVIB (#STC-410) action on CaV2.2 maximum current amplitude. Current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage ramp to +50 mV in 2 mM Ba2+. 10 nM ω-Conotoxin CVIB were perfused as indicated by the bar (green) for 5 min. B. Superimposed examples of CaV2.2 channel maximum peak current in the absence (control) and presence (green) of 10 nM ω-Conotoxin CVIB (taken from the experiment in A).
    References - Scientific background
    • 1. Lewis, R.J. et al. (2000) J. Biol. Chem. 275, 35335.
      • ω-Conotoxin CVIB is a peptide toxin originally isolated from the cat cone (Conus Catus). It is a blocker of voltage-gated N-,P- and Q-type CaV channels. CVIB has an effective concentration of 1-10 nM with an IC50 of 7.7 nM for N-type channels and 11 nM for P- and Q-type channels. Compared to other ω-conotoxins, CVIB is a fairly poor inhibitor of N-type channels and only a moderate inhibitor of P- and Q-type channels. Cloning and sequencing of mRNA transcripts from the venom ducts of the sea-snail failed to isolate CVIB’s mRNA transcript thus suggesting that this ω-conotoxin is not found abundantly in the Conus Catus species and may be found in other species of the cone snail family1.
    Target N-, P/Q-type Ca2+ channels
    Net Peptide Content: 100%
    Last update: 20/08/2019

    ω-Conotoxin CVIB (#STC-410) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use