ω-Conotoxin CVIC

A Blocker of N-, P/Q-Type Ca2+ Channels
    Cat #: STC-420
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 2783 Da.
    Purity: >99% (HPLC)
    Effective concentration 10-50 nM.
    Sequence CKGKGQSCSKLMYDCCTGSCSRRGKC.
    Modifications Disulfide bonds between Cys1-Cys16, Cys8-Cys20, and Cys15-Cys26. Ile26 – C-terminal amidation.
    Structure
    • ω-Conotoxin CVIC
    Molecular formula C107H180N38O35S7.
    Activity ω-Conotoxin CVIC is a blocker of N-, P/Q-type CaV channels1.
    References-Activity
    1. Lewis, R.J. et al. (2000) J. Biol. Chem. 275, 35335.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10,000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • ω-Conotoxin CVIC
      Alomone Labs ω-Conotoxin CVIC inhibits CaV2.2 channels expressed in Xenopus oocytes.
      A. Time course of ω-Conotoxin CVIC (#STC-420) action on CaV2.2 maximum current amplitude. Current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage ramp to +50 mV in 2 mM Ba2+. 50 nM ω-Conotoxin CVIC were perfused as indicated by the bar (green) for 5 min. B. Superimposed examples of CaV2.2 channel maximum peak current in the absence (control) and presence (green) of 50 nM ω-Conotoxin CVIC (taken from the experiment in A).
    References - Scientific background
    1. Lewis, R.J. et al. (2000) J. Biol. Chem. 275, 35335.
    Scientific background ω-Conotoxin CVIC is a peptide toxin originally isolated from the cat cone (Conus Catus). It is a blocker of voltage-gated N-,P- and Q-type CaV channels. CVIC has an effective concentration of 10-50 nM with an IC50 of 7.6 nM for N-type channels and 31 nM for P- and Q-type channels. Compared to other ω-conotoxins, CVIC is a fairly poor inhibitor of N-type channels and only a moderate inhibitor of P- and Q-type channels. Cloning and sequencing of mRNA transcripts from the venom ducts of the sea-snail failed to isolate CVIC’s mRNA transcript thus suggesting that this ω-conotoxin is not found abundantly in the Conus Catus species and may be found in other species of the cone snail family1.
    Target N-, P/Q-type channels
    Net Peptide Content: 100%
    Last update: 24/01/2020

    ω-Conotoxin CVIC (#STC-420) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use
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