ω-Conotoxin GVIA

SNX-124
A Blocker of N-Type Ca2+ Channels
    Cat #: C-300
    Alternative Name SNX-124
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 3037 Da.
    Purity: >99% (HPLC)
    Effective concentration 20 nM - 1 μM.
    Sequence CKSPGSSCSPTSYNCCRSCNXYTKRCY.
    Modifications Disulfide bonds between Cys1-Cys16, Cys8-Cys19 and Cys15-Cys26. X = Hydroxyproline. Tyr27 - C-terminal amidation.
      • ω-Conotoxin GVIA
    Molecular formula C120H188N38O43S6.
    CAS No.: 106375-28-4.
    Activity ω-Conotoxin GVIA specifically and reversibly blocks N-type CaV channels1 and is reported to antagonize P2X receptors2.
      • McCleskey, E.W. et al. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 4327.
      • Lalo, U.V. et al. (2001) Brain Res. Bull. 54, 507.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • ω-Conotoxin GVIA
        Alomone Labs ω-Conotoxin GVIA inhibits CaV2.2 heterologously expressed in Xenopus oocytes.
        Left: Time course of ω-Conotoxin GVIA (#C-300) action. Current amplitudes were plotted as a function of the stimulus number. Oocyte membrane potential was held at –100 mV and 100 ms stimulating pulse to 0 mV was delivered every 10 seconds. 20 nM ω-Conotoxin GVIA was perfused in the period marked by the bar. Right: Example traces of N-type currents before and during ω-Conotoxin GVIA application.
    References - Scientific background
      • ω-Conotoxin GVIA is a synthetic toxin originally isolated from the Conus geographus. ω-Conotoxin GVIA is a specific blocker of CaV2.2 Ca2+ channels. It specifically blocks N-type CaV channels by binding to the CaV2.2 α1 subunit (α1B) and its action is only partially reversible.2,3 In accordance, it inhibits synaptic transmission in many systems.4 It is also reported to antagonize P2X receptors.5 The toxin is used to specifically investigate CaV2.2 channel's contributions (by subtraction of the activity before and during perfusion of the toxin). Alternatively, it is used to eliminate the N-type channel contribution to highlight some other channel or enzyme activity. ω-Conotoxin GVIA was also used to purify the channel protein using immunoprecipitation techniques and to label and localize channels and synapses.6

    Target N-type Ca2+ channels
    Net Peptide Content: 100%
    Image & Title ω-Conotoxin GVIA
    Alomone Labs ω-Conotoxin GVIA blocks CaV2.2 channel in mouse Calyx of Held.Calyxes were whole-cell voltage-clamped at -80 mV. Traces were recorded in the presence of 1 mM CaCl2, pharmacological isolation of VGCC subtypes was performed in 2 mM CaCl2. 2 µM ω-Conotoxin GVIA (#C-300) was applied to selectively block CaV2.2.Adapted from Lubbert, M. et al. (2017) eLife 6, e28412. with permission of eLife Sciences.
    Last update: 08/09/2019

    ω-Conotoxin GVIA (#C-300) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use
    Citations
      • ω-Conotoxin GVIA
        Knockdown of FMRP enhances synaptic vesicle exocytosis in presynaptic terminals of DRG neurons via CaV2.2 channels.
        A and B. vGpH response to 40 AP at 10 Hz from presynaptic terminals of DRG neurons transfected with Ctrl shRNA (A) or FMRP shRNA (B) before and after treatment with ω-Conotoxin GVIA (#C-300) and ω-Agatoxin IVA (#STA-500). Fluorescence intensities were normalized to the peak of a brief application of NH4Cl. C. Normalized vGpH responses to 40 AP at 10 Hz from presynaptic terminals of DRG neurons transfected with Ctrl shRNA (black-filled bar, 100±10.6%, n = 38) or FMRP shRNA (red open bar, 137.0±12.6%, n = 25, P = 0.027). ω-Conotoxin GVIA (ConoTx) reduces Ctrl shRNA and FMRP shRNA responses to a similar level (44.7±4.9%, n = 15 and 41.6±3.3%, n = 24, respectively). ω-Conotoxin GVIA and ω-Agatoxin IVA (AgaTx) application further reduces the responses: Ctrl shRNA = 17.3±3.2%, n = 38, and FMRP shRNA = 18.1±5.0%, n = 27. D. Average vGpH response to a 40 Hz stimulation for 30 s from presynaptic terminals of DRG neurons transfected with Ctrl shRNA (blackfilled squares) or FMRP shRNA (open red squares).
        Adapted from Ferron, L. et al. (2014) with permission of Springer Nature.
      • Rat INS-1 832/12 cells (whole-cell calcium currents).
        Luan, C. et al. (2019) Commun. Biol. 2, 106.
      • Rat DRGs (patch clamp).
        Moutal, A. et al. (2018) Neuroscience 381, 79.
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