Overview
Type: Synthetic peptide
Form: Lyophilized powder
β2-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR016) is the original antigen used for immunization during Anti-β2-Adrenergic Receptor (extracellular) Antibody (#AAR-016) generation. The blocking peptide binds and ‘blocks’ Anti-β2-Adrenergic Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart (lanes 1 and 3) and lung (lanes 2 and 4) lysates:1,3. Anti-β2-Adrenergic Receptor (extracellular) Antibody (#AAR-016), (1:200).
2,4. Anti-β2-Adrenergic Receptor (extracellular) Antibody, preincubated with β2-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR016).
Expression of β2-Adrenoceptor in rat lungImmunohistochemical staining of paraffin-embedded section of rat lung using Anti-β2-Adrenergic Receptor (extracellular) Antibody (#AAR-016), (1:100). β2-Adrenoceptor is expressed in the respiratory epithelium. Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)NGSRAPDHDVTQERDE, corresponding to amino acid residues 15-30 of mouse ADRB2 (Accession P18762).
Accession (Uniprot) Number P18762
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
