1-EBIO

1-Ethyl-2-benzimidazolinone
A Potent Activator of KCa3.1 and KCa2 Channels
    Cat #: E-150
    Alternative Name 1-Ethyl-2-benzimidazolinone
  • Lyophilized Powder
  • Bioassay Tested
  • Source Synthetic
    MW: 162.19
    Purity: >97%
    Effective concentration 5-100 μM.
      • 1-EBIO
    Chemical name 3-ethyl-1H-benzimidazol-2-one.
    Molecular formula C9H10N2O.
    CAS No.: 10045-45-1.
    PubChem CID 82320
    Activity 1-EBIO is an activator of KCa3.1 and KCa2 channel currents1.
      • Waschk, D.E. et al. (2011) Am. J. Physiol. Cell. Physiol. 300, C843.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility 250 mM DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
      • 1-EBIO
        Alomone Labs 1-EBIO activates KCa3.1 currents expressed in HEK 293T cells.
        A. Time course of KCa3.1 current amplitude and its activation by 50 µM 1-EBIO (#E-150). Currents were elicited by application of voltage ramp from -120 mV to +60 mV (150 msec), delivered every 10 seconds, holding potential of -80mV. B. Superimposed example traces of current responses before and during perfusion of 50 µM 1-EBIO as indicated.
    References - Scientific background
    • 1. Wong, R. et al. (2014) J. Neurosci. 34, 13371.
    • 2. Coleman, N. et al. (2014) Mol. Pharmacol. 86, 342.
    • 3. Strøbæk, D. et al. (2013) Br. J. Pharmacol. 168, 432.
      • 1-EBIO is a potent KCa2/3 channel activator that activates the channels by increasing the Ca2+ sensitivity by stabilizing the interaction between calmodulin and the channels1,2, thus shifting the activation curve of the channels to the left. 1-EBIO activates KCa3.1 with an EC50 value of around 30 μM and all three KCa2 channels with EC50 value around 300 μM2.

        The Ca2+-activated K+ channel, KCa3.1, plays a major role in the controlled expression of essential genes that are responsible for several physiological processes including- membrane potential, cell volume proliferation, migration and production of inflammatory mediators by participating in the regulation of calcium entry. A highly regulated and sustained increase in cytosolic Ca2+ is critical in these processes making KCa3.1 a potential target for therapy of several disease1,3.

    Target KCa3.1, KCa2 channels
    Last update: 02/12/2019

    1-EBIO (#E-150) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use