1Kα-Pompilidotoxin

1Kα-PMTX
An Activator of NaV Channels
    Cat #: P-172
    Alternative Name 1Kα-PMTX
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 1502 Da.
    Purity: >98% (HPLC)
    Effective concentration 100-200 µM.
    Sequence KIKIGLFDQLSKL.
    Modifications Leu13 – C-terminal amidation.
    Structure
    • 1Kα-Pompilidotoxin
    Molecular formula C71H124N18O17.
    Activity 1Kα-PMTX slows NaV channel inactivation without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse1-3.
    References-Activity
    1. Schiavon, E. et al. (2010) FEBS J. 277, 918.
    2. Sahara, Y. et al. (2000) Eur. J. Neurosci. 12, 1961.
    3. Konno, K. et al. (2000) Neurosci. Lett. 285, 29.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer, pH > 8. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • 1Kα-Pompilidotoxin
      Alomone Labs 1Kα-Pompilidotoxin enhances native NaV currents in ND7-23 cells.
      Native TTX-sensitive NaV currents in ND7-23 cells were elicited by a 50 ms voltage ramp from the holding potential of -100 mV to +60 mV, applied every 10 sec using whole-cell voltage clamp. A. Time course, showing the effect of 200 µM 1Kα-Pompilidotoxin (#P-172) application (horizontal bar) on current area, indicative of a toxin-dependent decrease in NaV currents inactivation. B. Superimposed traces of NaV currents in ND7-23 cells under control conditions and after 2 min perfusion with 200 µM 1Kα-Pompilidotoxin (as indicated).
    • 1Kα-Pompilidotoxin
      Alomone Labs 1Kα-Pompilidotoxin enhances NaV1.6 currents expressed in Xenopus oocytes.
      A. Time course of 1Kα-Pompilidotoxin (#P-172) action on NaV1.6 currents. Current area was plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage step to 0 mV, applied every 10 sec using two electrode voltage clamp. 20 µM (red) or 100 µM (green) 1Kα-Pompilidotoxin were perfused as indicated by the horizontal bars. B.Superimposed traces of NaV1.6 currents in the absence (control) and presence of 20 µM (red) or 100 µM (green) 1Kα-Pompilidotoxin.
    References - Scientific background
    1. Schiavon, E. et al. (2010) FEBS J. 277, 918.
    2. Sahara, Y. et al. (2000) Eur. J. Neurosci. 12, 1961.
    3. Konno, K. et al. (2000) Neurosci. Lett. 285, 29.
    4. Konno, K. et al. (1998) Biochem Biophys. Res. Commun. 250, 612.
    5. Harsch, A. et al. (1998) Neurosci. Lett. 252, 49.
    Scientific background

    1Kα-PMTX is a mutated form of α-PMTX (#P-170), originally isolated from the solitary wasp (Anoplius samariensis) venom and is a synthetic version of the peptide1.

    Pompilidotoxins (PMTX, α and β) are small peptides consisting of 13 amino acids.

    α-PMTX induces a unique pattern of repetitive action potentials previously seen with other neurotoxins and thus facilitates both excitatory and inhibitory synaptic transmission.

    Recently it was found that this toxin slows the Na+-channel inactivation, without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse of the lobster walking leg and in the rat trigeminal ganglion neurons2-4. It is effective at concentrations of 10 nM in cultured rat cortical neurons5.

    1Kα-PMTX is mutated at position 1 with Arginine to Lysine transition, which enhances its activity on voltage-gated Na+ channels 5-fold relative to α-PMTX1,3.

    Target NaV Na+ channels
    Net Peptide Content: 100%
    Last update: 24/01/2020

    1Kα-Pompilidotoxin (#P-172) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use
    Shipping and Ordering information