1Kβ-Pompilidotoxin

1Kβ-PMTX
An Activator of NaV Channels
    Cat #: P-182
    Alternative Name 1Kβ-PMTX
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 1531 Da.
    Purity: >98% (HPLC)
    Effective concentration 100-200 µM.
    Sequence KIKIGLFDQLSRL.
    Modifications Leu13 – C-terminal amidation.
      • 1Kβ-Pompilidotoxin
    Molecular formula C71H124N20O17.
    Activity 1Kβ-PMTX slows NaV channel inactivation without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse1-3. 1Kβ-PMTX showed the largest effects on the various tested NaV isoforms, mainly on NaV1.6 channels1.
      • Schiavon, E. et al. (2010) FEBS J. 277, 918.
      • Sahara, Y. et al. (2000) Eur. J. Neurosci. 12, 1961.
      • Konno, K. et al. (2000) Neurosci. Lett. 285, 29.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer, pH > 8. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • 1Kβ-Pompilidotoxin
        Alomone Labs 1Kβ-Pompilidotoxin enhances native NaV currents in ND7-23 cells.
        Native TTX-sensitive NaV currents in ND7-23 cells were elicited by a 50 ms voltage ramp from the holding potential of -100 mV to +60 mV, applied every 10 sec using whole-cell voltage clamp. A. Time course, showing the effect of 200 µM 1Kβ-Pompilidotoxin (#P-182) application (horizontal bar) on current area, indicative of a toxin-dependent decrease in NaV currents inactivation. B. Superimposed traces of NaV currents in ND7-23 cells under control conditions and after 2 min perfusion with 200 µM 1Kβ-PMTX (as indicated).
        1Kβ-Pompilidotoxin
        Alomone Labs 1Kβ-Pompilidotoxin enhances NaV1.6 currents heterologously expressed in Xenopus oocytes.
        NaV1.6 currents were elicited by a 100 ms voltage step from a holding potential of -100 mV to 0 mV applied every 10 sec, using two electrode voltage clamp. A. Time course, showing the effect of 20 µM, 50 µM and 100 µM 1Κβ-Pompilidotoxin (#P-182) application (horizontal bars) on current area. B. Superimposed traces of NaV1.6 current under control conditions and after 2 min perfusion with 20 µM, 50 µM and 100 µM 1Κβ-Pompilidotoxin (as indicated).
    References - Scientific background
    • 1. Schiavon, E. et al. (2010) FEBS J. 277, 918.
    • 2. Sahara, Y. et al. (2000) Eur. J. Neurosci. 12, 1961.
    • 3. Konno, K. et al. (2000) Neurosci. Lett. 285, 29.
    • 4. Konno, K. et al. (1998) Biochem. Biophys. Res. Commun. 250, 612.
    • 5. Harsch, A. et al. (1998) Neurosci. Lett. 252, 49.
      • 1Kβ-PMTX is a mutated form of the β-PMTX (#P-180), originally isolated from the solitary wasp (Batozonellus maculifrons) venom and is a synthetic version of the peptide1. Pompilidotoxins (PMTX, α and β) are small peptides consisting of 13 amino acids.

        β-PMTX induces a unique pattern of repetitive action potentials previous seen with other neurotoxins and thus facilitates both excitatory and inhibitory synaptic transmission.

        Recently it was found that this toxin slows the Na+-channel inactivation, without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse of the lobster walking leg and in the rat trigeminal ganglion neurons2-4. It is effective at concentrations of 10 nM in cultured rat cortical neurons5.

        1Kβ-PMTX is mutated at position 1 with Arginine to Lysine transition, which enhances its activity on voltage-gated human Nav1.6 Na+ channels relative to native β-PMTX1,3.

    Target NaV Na+ channels
    Net Peptide Content: 100%
    Last update: 13/11/2019

    1Kβ-Pompilidotoxin (#P-182) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use