3Rα-Pompilidotoxin

3Rα-PMTX
An Activator of NaV Channels
Cat #: P-174
Alternative Name 3Rα-PMTX
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 1558 Da.
    Purity: >98% (HPLC)
    Effective concentration 100-200 µM.
    Sequence RIRIGLFDQLSKL.
    Modifications Leu13 – C-terminal amidation.
    Molecular formula C71H124N22O17.
    Activity 3Rα-Pompilidotoxin slows NaV channel inactivation without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse1-3.
      • Schiavon, E. et al. (2010) FEBS J. 277, 918.

      • Sahara, Y. et al. (2000) Eur. J. Neurosci. 12, 1961.

      • Konno, K. et al. (2000) Neurosci. Lett. 285, 29.

    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer, pH > 8. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • Alomone Labs 3Rα-Pompilidotoxin enhances native NaV currents in ND7-23 cells.
        Native TTX-sensitive NaV currents in ND7-23 cells were elicited by a 50 ms voltage ramp from the holding potential of -100 mV to +60 mV, applied every 10 sec using whole-cell voltage clamp. A. Time course, showing the effect of 100 µM 3Rα-Pompilidotoxin (#P-174) application (horizontal bar) on current area, indicative of a toxin-dependent decrease in NaV currents inactivation. B. Superimposed traces of NaV currents in ND7-23 cells under control conditions and after 2 min perfusion with 100 µM 3Rα-Pompilidotoxin.
      • Schiavon, E. et al. (2010) FEBS J. 277, 918.
      • Sahara, Y. et al. (2000) Eur. J. Neurosci. 12, 1961.
      • Konno, K. et al. (2000) Neurosci. Lett. 285, 29.
      • Konno, K. et al. (1998) Biochem Biophys. Res. Commun. 250, 612.
      • Harsch, A. et al. (1998) Neurosci. Lett. 252, 49.
      • 3Rα-Pompilidotoxin is a mutated form of α-Pompilidotoxin (#P-170), originally isolated from the solitary wasp (Anoplius samariensis) venom and is a synthetic version of the peptide1.

        Pompilidotoxins (PMTX, α and β) are small peptides consisting of 13 amino acids.

        α-PMTX induces a unique pattern of repetitive action potentials previously seen with other neurotoxins and thus facilitate both excitatory and inhibitory synaptic transmission.

        Recently it was found that this toxin slows the Na+-channel inactivation, without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse of the lobster walking leg and in the rat trigeminal ganglion neurons2-4. Effective at concentrations of 10 nM in cultured rat cortical neurons5.

        3Rα-PMTX is mutated at position 3 with Lysine to Arginine transition, which enhances its activity on voltage-gated Na+ channels 5-fold relative to α-PMTX1,3.

    Target NaV Na+ channels
    Net Peptide Content: 100%
    Last update: 01/07/2019

    3Rα-Pompilidotoxin (#P-174) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use