Overview
Type: Synthetic peptide
Form: Lyophilized powder
5HT2A Receptor/HTR2A (extracellular) Blocking Peptide (#BLP-SR033) is the original antigen used for immunization during Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033) generation. The blocking peptide binds and ‘blocks’ Anti-5HT2A Receptor/HTR2A (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat DRG (lanes 1 and 4), mouse brain (lanes 2 and 5) and human brain neuroblastoma SH-SY5Y (lanes 3 and 6) lysates:1-3. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033), (1:200).
4-6. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody, preincubated with 5HT2A Receptor/HTR2A (extracellular) Blocking Peptide (#BLP-SR033).- Mouse trigeminal ganglion sections (1:500) (Chang, W. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. 113, E5491.).
Expression of 5HT2A Receptor in rat cingulate cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033), (1:1200), followed by goat anti-rabbit-AlexaFluor-488. A. 5HT2A Receptor immunoreactivity (green) appears in outlines of cortical neurons (arrows). B. Pre-incubation of the antibody with 5HT2A Receptor (HTR2A) (extracellular) Blocking Peptide (BLP-SR033), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of 5HT2A Receptor in rat dorsal raphe nucleus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033), (1:1200), followed by goat anti-rabbit-AlexaFluor-488. A. 5HT2A Receptor immunoreactivity (green) appears in outlines of neurons (arrows). B. Pre-incubation of the antibody with 5HT2A Receptor (HTR2A) (extracellular) Blocking Peptide (BLP-SR033), suppressed staining. Cell nuclei are stained with DAPI (blue).
Western blot analysis of rat dorsal root ganglion lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1, 2. Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033-GP), (1:200).
3, 4. Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody, preincubated with 5HT2A Receptor (HTR2A) (extracellular) Blocking Peptide (#BLP-SR033).
Expression of 5-HT2A in rat dorsal raphe nucleus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033-GP), (1:400), followed by goat anti-guinea pig - Alexa Fluor-594. A. 5-HT2A immunoreactivity (red) appears in neuronal outlines (arrows). B. Pre-incubation of the antibody with 5HT2A Receptor (HTR2A) (extracellular) Blocking Peptide (#BLP-SR033), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)SEASNWTIDAENR, corresponding to amino acid residues 40-52 of rat 5-Hydroxytryptamine receptor 2A (Accession P14842).
Accession (Uniprot) Number P14842
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
