Overview
Type: Synthetic peptide
Form: Lyophilized powder
5HT4 Receptor/HTR4 (extracellular) Blocking Peptide (#BLP-SR036) is the original antigen used for immunization during Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (#ASR-036) generation. The blocking peptide binds and ‘blocks’ Anti-5HT4 Receptor/HTR4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (#ASR-036), (1:200).
2. Anti-5HT4 Receptor (HTR4) (extracellular) Antibody, preincubated with 5HT4 Receptor/HTR4 (extracellular) Blocking Peptide (#BLP-SR036).
Properties
Sequence
- (C)DVIEKRKFNHNSN, corresponding to amino acid residues 168-180 of rat 5-Hydroxytryptamine receptor 4 (Accession Q62758).
Accession (Uniprot) Number Q62758
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
