A 967079

A Blocker of TRPA1 Channels
    Cat #: A-225
  • Lyophilized Powder
  • Bioassay Tested
  • Source Synthetic
    MW: 207.2
    Purity: >99% (HPLC)
    Form Lyophilized powder.
    Effective concentration 10 nM - 100 µM.
    Structure
    • A 967079
    Chemical name (NZ)-N-[(E)-1-(4-fluorophenyl)-2-methylpent-1-en-3-ylidene]hydroxylamine.
    Molecular formula C12H14FNO.
    CAS No.: 1170613-55-4.
    Activity A 967079 is a blocker of TRPA1 channels and has IC50 values of 67 and 289 nM at human and rat TRPA1 receptors respectively1.
    References-Activity
    1. McGaraughty, S.  et al. (2010) Mol. Pain 6, 14.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Up to 200 mM in DMSO. Centrifuge all product preparations before use (5,000 x g for 1 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
    Our bioassay
    • A 967079
      Alomone Labs A 967079 inhibits Allyl isothiocyanate induced calcium signal in TRPA1 expressing cells. 
      Dose-response curve for the inhibition of human TRPA1 expressing HEK-293 cells loaded with Calcium 5 dye for changes in intracellular Ca2+ measurements. Allyl isothiocyanate (100 µM)-induced calcium signal was completely inhibited by 0.5 µM A 967079 (#A-225), with an estimated IC50 of about 120 nM.
    References - Scientific background
    1. Levine, J.D. and Alessandri-Haber, N. (2007) Biochim. Biophys. Act. 1772, 989.
    2. Bautista, D.M. (2006) Cell 124, 1269.
    3. Bessac, B.F. and Jordt, S. (2008) Physiology 23, 360.
    Scientific background

    The transient receptor potential (TRP) channel is one of the largest families of cation channels1.

    TRPA1 is expressed in sensory and nociceptive neurons and has a significant role in the inflammatory response. Bradykinin and histamine receptors induce TRPA1 activity. It has been found that TRPA1 deficient mice do not develop an elevated mechanical and thermal pain response following bradykinin injection. This suggests that TRPA1 has a vital role in bradykinin signaling pathways2.

    TRPA1 also plays a role in acute and chronic respiratory disease pathophysiology. Prolonged exposure to TRPA1 agonists such as chlorine and aldehydes can cause reactive airway dysfunction syndrome characterized by symptoms such as wheezing, cough and dyspnea. In view of this, TRPA1 is a potential target in airway anti-inflammatory research3.

    A 967079 is a TRPA1 blocker and has an IC50 value of 67 and 289 nM for human and rat TRPA1 receptors respectively.

    Target TRPA1 channels
    Last update: 05/06/2020

    A 967079 (#A-225) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use
    Shipping and Ordering information