Overview
Type: Synthetic peptide
Form: Lyophilized powder
Adenosine A2A Receptor Blocking Peptide (#BLP-AR002) is the original antigen used for immunization during Anti-Adenosine A2A Receptor Antibody (#AAR-002) generation. The blocking peptide binds and ‘blocks’ Anti-Adenosine A2A Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat aortic endothelial cells (lanes 1, 3), rat brain (lanes 2, 4) and Jurkat (lanes 5, 6) lysates:1,2,5. Anti-Adenosine A2A Receptor Antibody (#AAR-002), (1:200).
3,4,6. Anti-Adenosine A2A Receptor Antibody, preincubated with Adenosine A2A Receptor Blocking Peptide (#BLP-AR002).
Expression of Adenosine A2A Receptor in mouse diagonal bandImmunohistochemical staining of mouse diagonal band using Anti-Adenosine A2A Receptor Antibody (#AAR-002). A2aR (green) appears in the broca of individual neurons (arrows) and in neuropil (asterisk). DAPI is used as the counterstain.
Expression of Adenosine A2A Receptor in rat lungImmunohistochemical staining of paraffin emedded rat lung sections using Anti-Adenosine A2A Receptor Antibody (#AAR-002), (1:50). A2aR is expressed in the respiratory epithelium of the bronchioli (Br). Note that smooth muscle and endothelium in blood vessels are negative. Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)RQLKQMESQPLPGER, corresponding to amino acid residues 201-215 of mouse adenosine A2A receptor (Accession Q60613).
Accession (Uniprot) Number Q60613
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
