Adenosine A3 Receptor Blocking Peptide (#BLP-AR004) is the original antigen used for immunization during Anti-Adenosine A3 Receptor Antibody (#AAR-004) generation. The blocking peptide binds and ‘blocks’ Anti-Adenosine A3 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of Malme-3M cell lysate:1. Anti-Adenosine A3 Receptor Antibody (#AAR-004), (1:200).
2. Anti-Adenosine A3 Receptor Antibody, preincubated with Adenosine A3 Receptor Blocking Peptide (#BLP-AR004).
- Expression of Adenosine A3 Receptor in rat lungImmunohistochemical staining of paraffin-embedded rat lung sections using Anti-Adenosine A3 Receptor Antibody (#AAR-004), (1:50). Staining is specific for respiratory epithelial cells of the bronchiole (arrows) and pneumonocytes in the alveolar wall (a). Hematoxilin is used as the counterstain.
- (C)KETGAFYGREFKTAK, corresponding to amino acid residues 216-230 of human A3AR (Accession P0DMS8).