Cat #: RTA-390
Alternative Name K+ channel toxin α-KTx 3.3, AgTx-3, AgTx3
This product is freeze dried. All water molecules have been removed.
Every lot is tried & tested in a relevant biological assay.
Origin Recombinant, E. coli
MW: 4101 Da.
Purity: >98% (HPLC)
Effective concentration 50 pM - 10 nM.
Modifications Disulfide bonds between Cys8-Cys28, Cys14-Cys33, and Cys18-Cys35.
Molecular formula C171H280N54O47S8.
CAS No.: 155646-23-4.
Activity Agitoxin-3 is a blocker of Shaker voltage-gated K+ channels as well as the mammalian homologues of Shaker.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to one week at 4°C or three months at -20°C.
- Alomone Labs Agitoxin-3 inhibits KV1.3 channels heterologously expressed in Xenopus oocytes.Left: Time course of KV1.3 current amplitude in response to test pulse of 100 ms to 0 mV (holding potential -100 mV). 20 nM Agitoxin-3 (#RTA-390) were perfused at the period marked by the horizontal bar. Right: Traces of KV1.3 currents before (red) and during (black) application of the toxin.
References - Scientific background
- 1. Garcia, M.L. et al. (1994) Biochemistry 33, 6834.
- Native Agitoxin-3 was originally isolated from the venom of the Israeli scorpion L. quinquestriatus hebraeus.1 Like Agitoxin-2 (#STA-420), it is a potent blocker of the Shaker voltage-gated K+ channels as well as the mammalian homologues of Shaker.1
Target KV1.3 K+ channels
Net Peptide Content: 100%
Last update: 13/11/2019
Agitoxin-3 (#RTA-390) is a highly pure, recombinant, and biologically active peptide toxin.
For research purposes only, not for human use