Alpha 3 Na+/K+ ATPase Blocking Peptide (#BLP-NP003) is the original antigen used for immunization during Anti-Alpha 3 Na+/K+ ATPase Antibody (#ANP-003) generation. The blocking peptide binds and ‘blocks’ Anti-Alpha 3 Na+/K+ ATPase primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
- Expression of Na+/K+ ATPase α3 (ATP1A3) in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Alpha 3 Na+/K+ ATPase Antibody (#ANP-003), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ATP1A3 staining (green) in the hippocampal dentate gyrus is detected in the granule layer (G), (vertical arrows) and in hilar interneurons (horizontal arrows). Cell nuclei are stained with DAPI (blue).
- (C)DKKDDKSSPKKSK, corresponding to amino acid residues 3-15 of rat ATP1A3 (Accession P06687).