AMG-9090

A Blocker of TRPA1, TRPV1 and TRPM8 Channels
    Cat #: A-300
  • Lyophilized Powder
  • Bioassay Tested
  • Source Synthetic
    MW: 395.12
    Purity: >98%
    Effective concentration 50 nM - 50 µM.
    Structure
    • AMG-9090
    Chemical name N-[2,2,2-trichloro-1-(4-chlorophenyl)sulfanylethyl]benzamide.
    Molecular formula C15H11Cl4NOS.
    CAS No.: 118215-82-0.
    PubChem CID 3277297
    Activity AMG-9090 is TRPA1 channel blocker, shown to potently and concentration-dependently inhibit AITC-induced increase in intracellular calcium mediated by human TRPA1 (IC50 = 21 nM)1,2. The compound also possesses inhibitory effects on human TRPM8 and TRPV1 channels1,2.
    References-Activity
    1. Klionsky, L. et al. (2007) Mol. Pain 3, 39.
    2. Miller, S. et al. (2014) PLoS ONE 9, e107151.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. Protect from light.
    Solubility Up to 50 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).
    Storage of solutions Up to three months at -20°C.
    Our bioassay
    • AMG-9090
      Alomone Labs AMG-9090 inhibits TRPA1 channel currents in HEK-293 cells.
      Dose-response curve of AMG-9090 (#A-300) inhibition of human TRPA1-mediated increase in intracellular Ca2+. Cells were loaded with Calcium 5 dye, incubated for 10 min with AMG-9090, and stimulated by 1 µM allyl isothiocyanate (EC80). Changes in intracellular Ca2+ following stimulation were detected as changes in maximum relative fluorescence (RFU). IC50 was determined at 0.527 nM.
    References - Scientific background
    1. Klionsky, L. et al. (2007) Mol. Pain 3, 39.
    2. Miller, S. et al. (2014) PLoS ONE 9, e107151.
    3. Benemei, S. et al. (2013) J. Headache Pain 14, 71.
    Scientific background

    AMG-9090 is a small synthetic molecule that acts as a TRPA1 channel blocker. AMG-9090 was shown to inhibit intracellular calcium increase mediated by human TRPA1 channels in Chinese hamster ovary cells with IC50 values of 21 nM1. The compound also possesses inhibitory effects on human TRPM8 and TRPV1 channels1,2.

    TRPA1 channels are non-selective cation channels expressed in the small neurons of the dorsal root and trigeminal ganglia. They play an important role in models of inflammatory and neuropathic pain and have the ability to detect pungent or irritant compounds.

    Studies have shown that blockade of TRPA1 channels may help in migraine attack and can be used as a possible new target for its treatment. Also, evidence shows that TRPA1 blockers could serve as novel therapeutic strategies for chemotherapeutic induced peripheral neuropathy1,3.

    Target TRPA1, TRPM8, TRPV1 channels
    Last update: 24/01/2020

    AMG-9090 (#A-300) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use
    Shipping and Ordering information