Name: AMG-9090
Brand: Alomone Labs
SKU: A-300

Cat #: A-300

Lyophilized Powder yes

Bioassay Tested

Source Synthetic

MW: 395.12

Purity: >98%

Effective concentration 50 nM - 50 µM.

Structure

Chemical name N-[2,2,2-trichloro-1-(4-chlorophenyl)sulfanylethyl]benzamide.

Molecular formula C₁₅H₁₁Cl₄NOS.

CAS No.: 118215-82-0

PubChem CID 3277297

Activity AMG-9090 is TRPA1 channel blocker, shown to potently and concentration-dependently inhibit AITC-induced increase in intracellular calcium mediated by human TRPA1 (IC₅₀ = 21 nM)^1,2. The compound also possesses inhibitory effects on human TRPM8 and TRPV1 channels^1,2.

References-Activity

Klionsky, L. et al. (2007) Mol. Pain 3, 39.
Miller, S. et al. (2014) PLoS ONE 9, e107151.

Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. Protect from light.

Solubility Up to 50 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).

Storage of solutions Up to three months at -20°C.

Our bioassay

Alomone Labs AMG-9090 inhibits TRPA1 channel currents in HEK-293 cells.
Dose-response curve of AMG-9090 (#A-300) inhibition of human TRPA1-mediated increase in intracellular Ca²⁺. Cells were loaded with Calcium 5 dye, incubated for 10 min with AMG-9090, and stimulated by 1 µM allyl isothiocyanate (EC₈₀). Changes in intracellular Ca²⁺ following stimulation were detected as changes in maximum relative fluorescence (RFU). IC₅₀ was determined at 0.527 nM.

References - Scientific background

Klionsky, L. et al. (2007) Mol. Pain 3, 39.
Miller, S. et al. (2014) PLoS ONE 9, e107151.
Benemei, S. et al. (2013) J. Headache Pain 14, 71.

Scientific background

AMG-9090 is a small synthetic molecule that acts as a TRPA1 channel blocker. AMG-9090 was shown to inhibit intracellular calcium increase mediated by human TRPA1 channels in Chinese hamster ovary cells with IC₅₀ values of 21 nM¹. The compound also possesses inhibitory effects on human TRPM8 and TRPV1 channels^1,2.

TRPA1 channels are non-selective cation channels expressed in the small neurons of the dorsal root and trigeminal ganglia. They play an important role in models of inflammatory and neuropathic pain and have the ability to detect pungent or irritant compounds.

Studies have shown that blockade of TRPA1 channels may help in migraine attack and can be used as a possible new target for its treatment. Also, evidence shows that TRPA1 blockers could serve as novel therapeutic strategies for chemotherapeutic induced peripheral neuropathy^1,3.

Target TRPA1, TRPM8, TRPV1 channels

Lyophilized Powder

Last update: 06/11/2022

AMG-9090 (#A-300) is a highly pure, synthetic, and biologically active compound.

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AMG-9090

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