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Certificate of Analysis

AMPA Receptor 1 Premium Research Pack

All You Need for AMPA Receptor 1 Research

Cat #: ESP-700
Size: 8 Vials
Form: Lyophilized

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-GluR1 (GluA1) (extracellular) Antibody Cat #: AGC-004

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide RTSDSRDHTRVDWKR(C), corresponding to amino acid residues 271-285 of rat GluR1 (Accession P19490). Extracellular, N-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 12/15 amino acid residues identical.

Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody Cat #: AGP-009

Size: 0.2 ml Host: Guinea pig Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide RTSDSRDHTRVDWKR(C), corresponding to amino acid residues 271-285 of rat GluR1 (Accession P19490). Extracellular, N-terminus.
Homology
Mouse - identical; human, pig, dog - 13/14 amino acid residues identical.

Pharmacological Reagents

(RS)-AMPA hydrobromide Cat #: A-266

Size: 10 mg Source: Synthetic MW: 267.07 Purity: >95%
Effective concentration
0.1-100 µM.
Activity
(RS)-AMPA is a selective AMPA receptor agonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
50 mM in H2O. Centrifuge all product preparations before use (10,000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

L-Quisqualic acid Cat #: L-210

Size: 10 mg Source: Synthetic MW: 189.1 Purity: 98%
Effective concentration
0.01-100 µM.
Activity
L-Quisqualic acid is a selective AMPA receptor agonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
20 mM in H2O. Centrifuge all product preparations before use (10 000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

CNQX Cat #: C-140

Size: 25 mg Source: Synthetic MW: 232.2 Purity: >98%
Effective concentration
300 nM - 500 µM.
Activity
AMPA/kainate receptor antagonist. The concentrations that inhibit 50% of AMPA binding (IC50) for CNQX in dorsal horn neurons and motoneurons are 300 nM-1.3 µM. It was about one-fifth as effective at kainate binding sites1,2. The association of AMPA receptors with TARPs converts CNQX from an antagonist to a weak partial agonist. CNQX binds with high affinity to both high and low affinity AMPA binding sites in rat brain. CNQX is a potent antagonist in electrophysiological responses mediated by non-NMDA receptors. Effects at NMDA receptors are much weaker.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g for 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Naspm trihydrochloride Cat #: N-215

Size: 25 mg Source: Synthetic MW: 479.91 Purity: >95% (HPLC)
Effective concentration
300 nM - 10 µM.
Activity
Naspm trihydrochloride is a selective AMPA receptor antagonist; ~0.3 uM IC50 for AMPA induced currents in rat hipocampal neurons1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
50 mM in H2O. Centrifuge all product preparations before use (10000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

NBQX disodium salt Cat #: N-186

Size: 25 mg Source: Synthetic MW: 380.2 Purity: >99%
Effective concentration
0.1- 500 µM.
Activity
NBQX is a potent inhibitor of non-NMDA glutamate receptor channels exhibiting IC50s of 0.1-0.4 μM for AMPA and 1.7-8 μM for kainate receptors1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

IDRA 21 Cat #: I-140

Size: 25 mg Source: Synthetic MW: 232.7 Purity: >99%
Effective concentration
10-500 µM.
Activity
IDRA 21 is a selective AMPA receptor agonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

Note

A guinea pig polyclonal antibody (#AGP-009) is included in this Research Pack. Please take into account when reacting with a secondary antibody.

AMPA Receptor 1 Overexpressed Membrane Fractions includes:
1 x 0.1 ml lyophilized AMPA receptor 1 overexpressed membrane fractions
1 x 0.1 ml lyophilized non-injected Xenopus oocyte membrane fractions

For research purposes only, not for human use
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