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AMTB hydrochloride

A Blocker of TRPM8 Channels

Cat #: A-305
Lyophilized Powder yes
  • Bioassay Tested
  • Source Synthetic
    MW: 430.99
    Purity: >97%
    Effective concentration 1-100 µM.
    Structure
    Chemical name N-(3-aminopropyl)-2-[(3-methylphenyl)methoxy]-N-(thiophen-2-ylmethyl)benzamide;hydrochloride.
    Molecular formula C23H27ClN2O2S.
    CAS No.: 926023-82-7
    PubChem CID 16095383
    Activity AMTB hydrochloride is a TRPM8 channel blocker, inhibiting icilin-induced human TRPM8 channel activation (pIC50 = 6.23), selective over TRPV1 and TRPV41.
    References-Activity
    1. Lashinger, E.S. et al. (2008) Am. J. Renal Physiol. 295, F803.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored desiccated at -20°C.
    Solubility Up to 100 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).
    Storage of solutions Up to three months at -20°C.
    Our bioassay
    • Alomone Labs AMTB hydrochloride inhibits TRPM8 channels expressed in HEK-293 cells.
      Alomone Labs AMTB hydrochloride inhibits TRPM8 channels expressed in HEK-293 cells.
      Dose-response curve of TRPM8 inhibition by AMTB hydrochloride (#A-305), showing complete inhibition at 100 µM (pIC50 = 5.11). Cells were loaded with Calcium-5 dye, incubated for 10 min with increasing concentrations of AMTB hydrochloride, and activated by 30 nM icilin. Changes in intracellular Ca2+ following agonist application were detected as changes in 485 nm/525 nm fluorescence (RFU).
    References - Scientific background
    1. Lashinger, E.S. et al. (2008) Am. J. Renal Physiol. 295, F803.
    2. Morgan, K. et al. (2014) Biosci. Rep. 34, 4.
    3. Behrendt, H.J. et al. (2004) Br. J. Pharmacol. 141, 737.
    4. Tsumura, M. et al. (2013) PLoS One 8, e82233.
    Scientific background

    AMTB hydrochloride is a TRPM8 channel blocker, able to inhibit icilin-induced human TRPM8 channel activation. The compound shows very little affinity for TRPV1 and TRPV4 channels and is being used as a tool to evaluate the role of TRPM8 channels in different animal models1. TRPM8 channel blockers may provide a new therapeutic treatment for overactive and painful bladder syndrome1,2.

    TRPM8 channel is a member of the TRP channel superfamily. TRPM8 is a Ca2+-permeable, nonselective cation channel that is activated by temperatures in the range of 8–28°C and by chemical agonists such as menthol and icilin. The channel is specifically expressed in temperature-sensing trigeminal and dorsal root ganglion neurons. It’s expression is up-regulated in prostate cancer and other malignancies3,4.

    Target TRPM8 channel
    Last update: 11/04/2021

    AMTB hydrochloride (#A-305) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use
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