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Certificate of Analysis

Angiotensin II Receptor Basic Research Pack

All You Need for Angiotensin II Receptor Research

Cat #: ESB-950
Size: 5 Vials
Form: Lyophilized

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody Cat #: AAR-011

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor (Accession P30556). Extracellular, N-terminus.
Homology
Mouse, rat, rabbit, dog - identical.

Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody Cat #: AAR-012

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide DNLNATGTNESAFNC, corresponding to amino acid residues 21-35 of rat AT2 receptor (Accession P35351). Extracellular, N-terminal domain.
Homology
Mouse - identical.

Pharmacological Reagents

Angiotensin A (1-8) Cat #: SPA-105

Size: 10 mg MW: 1002 Da Purity: ≥98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
0.5-1 nM.
Activity
An Angiotensin II Type 1 and Type 2 Receptor Agonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Angiotensin II acetate Cat #: SPA-165

Size: 50 mg MW: 1046 Da Purity: ≥98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1-300 nM.
Activity
An Angiotensin II Type 1 and Type 2 Receptor Agonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

PD 123319 ditrifluoroacetate Cat #: P-245

Size: 25 mg Source: Synthetic MW: 736.66 Purity: ≥97%
Effective concentration
1 nM – 10 µM.
Activity
An Angiotensin II Type 2 Receptor Antagonist1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100 mM in H2O. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

For research purposes only, not for human use
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