Certificate of Analysis

Angiotensin II Receptor Basic Research Pack

All You Need for Angiotensin II Receptor Research
Cat #: ESB-950 8 Vials

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody Cat #: AAR-011

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor (Accession P30556). Extracellular, N-terminus.
Homology
Mouse, rat, rabbit, dog - identical.

Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody Cat #: AAR-012

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide DNLNATGTNESAFNC, corresponding to amino acid residues 21-35 of rat AT2 receptor (Accession P35351). Extracellular, N-terminal domain.
Homology
Mouse - identical.

Pharmacological Reagents

Angiotensin A (1-8) Cat #: SPA-105

Size: 10 mg MW: 1002 Da. Purity: ≥98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
0.5-1 nM.
Activity
An Angiotensin II Type 1 and Type 2 Receptor Agonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Angiotensin II acetate Cat #: SPA-165

Size: 50 mg MW: 1046 Da. Purity: ≥98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1-300 nM.
Activity
An Angiotensin II Type 1 and Type 2 Receptor Agonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Azilsartan Cat #: A-245

Size: 50 mg Source: Synthetic MW: 456.45 Purity: ≥99%
Effective concentration
1-100 nM.
Activity
Azilsartan is an angiotensin II type 1 receptor antagonist. The physiologic effect of azilsartan is by means of decreased blood pressure1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
200 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

PD 123319 ditrifluoroacetate Cat #: P-245

Size: 25 mg Source: Synthetic MW: 736.66 Purity: ≥97%
Effective concentration
1 nM – 10 µM.
Activity
An Angiotensin II Type 2 Receptor Antagonist1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100 mM in H2O. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.