Anti-µ-Opioid Receptor/OPRM1 (extracellular)-FITC Antibody

Mu-type opioid receptor, Opioid receptor B, MOR-1, MOP, Mu-opiate receptor
  • New
Cat #: AOR-011-F
Alternative Name Mu-type opioid receptor, Opioid receptor B, MOR-1, MOP, Mu-opiate receptor
  • Lyophilized Powder
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CSPAPGSWLNLSHVDGN, corresponding to amino acid residues 22-38 of the rat µ-Opioid receptor (Accession P33535). Extracellular, N-terminus.
    • Anti-µ-Opioid Receptor/OPRM1 (extracellular)-FITC Antibody
    Accession (Uniprot) Number P33535
    Gene ID 25601
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human – 15/17 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Label Fluorescein isothiocyanate (FITC)
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AOR-011), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic*, lci*
    Direct flow cytometry
    • Anti-µ-Opioid Receptor/OPRM1 (extracellular)-FITC Antibody
      Cell surface detection of µ-Opioid Receptor in live intact mouse BV-2 microglia cells:
      ___ Cells.  
      ___ Cells + rabbit IgG isotype control-FITC.  
      ___ Cells + Anti-µ-Opioid Receptor/OPRM1 (extracellular)-FITC Antibody (#AOR-011-F), (2.5 µg).
    References
    1. Pan, L. et al. (2005) Neuroscience 133, 209.
    2. http://www.guidetopharmacology.org/.
    3. Matthes, H.W. et al. (1998) J. Neurosci. 18, 7285.
    4. Shaqura, M.A. et al. (2004) J. Pharmacol. Exp. Ther308, 712.
    5. Baraldi, P.G. et al. (2006) Curr. Med. Chem. 13, 3467.
    Scientific background

    Endogenous opiates such as endorphins, endomorphins, and enkephalins, as well as opiate drugs (including morphine) exert their effects by binding to opioid receptors. Three "classic" types of opioid receptors have been identified: mu (µ)-opioid (MOP) receptor, delta (δ)-opioid (DOP) receptor, and kappa (κ)-opioid (KOP) receptor1. Recently, the nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptor was also described. Despite its significant sequence homology, its pharmacological profile differs greatly from those of the classic µ, δ, and κ receptors2.

    The opioid receptors belong to the G protein-coupled receptor (GPCR) superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important for binding G proteins1.

    All three receptors mediate opioid-induced analgesia. Supraspinal analgesia is mainly mediated by the µ-receptors, whereas µ-, δ-, and κ-receptors participate in the control of pain at the spinal level. These receptors also mediate the mood-altering properties of opioids3.

    Of the opioid receptors, the µ-opioid receptor has been the most extensively studied due to its important role in mediating the actions of morphine and other analgesic agents, as well as other addictive drugs such as heroin1. The µ-opioid receptors are expressed in the central nervous system (CNS) and in the peripherial nervous system4. The highest densities are found in the thalamus, caudate putamen, neocortex, amygdala, and other brain regions known to have well established roles in pain and analgesia5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 15/06/2020

    Anti-µ-Opioid Receptor/OPRM1 (extracellular) Antibody (#AOR-011) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect live cell flow cytometry applications. It has been designed to recognize MOR-1 from rat, human, and mouse samples.

    Anti-µ-Opioid Receptor/OPRM1 (extracellular)-FITC Antibody (#AOR-011-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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