Free shipping starts now, no minimum, no coupons required!

Anti-α2A-Adrenergic Receptor (extracellular) Antibody

Alpha-2A adrenoceptor, Alpha-2D adrenergic Receptor, α-2AAR, ADA2A, ADRA2A

Cat #: AAR-020
Alternative Name Alpha-2A adrenoceptor, Alpha-2D adrenergic Receptor, α-2AAR, ADA2A, ADRA2A
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)DAGNSSWNGTEAPG, corresponding to amino acid residues 7-20 of rat α2A-adrenoceptor (Accession P22909). Extracellular, N-terminus.
Accession (Uniprot) Number P22909
Gene ID 25083
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 13/14 amino acid residues identical.
RRID AB_10687546.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, wb
May also work in: ic*, ip*, lci*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1, 2. Anti-α2A-Adrenergic Receptor (extracellular) Antibody (#AAR-020), (1:200).
    3, 4. Anti-α2A-Adrenergic Receptor (extracellular) Antibody, preincubated with α2A-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR020).
Immunohistochemistry
  • Expression of α2A-Adrenoreceptor in rat cerebellum
    Expression of α2A-Adrenoreceptor in rat cerebellum
    Immunohistochemical staining of rat cerebellum free floating frozen section using Anti-α2A-Adrenergic Receptor (extracellular) Antibody (#AAR-020). A. α2A-Adrenoreceptor staining (green) appears in processes of Bergmann glia (white arrows) in the molecular layer (ML). B. Staining of the same section with mouse anti glial fibrillary acidic protein (GFAP) (red), demonstrates expression in both Bergmann glia and in granule layer (GL) astrocytes. C. Merged image of panel A and B demonstrating that α2A-adrenoreceptor expression is restricted to Bergmann glia. DAPI is used as the counterstain (blue).
  • Expression of α2A-Adrenoreceptor in rat hypothalamus
    Expression of α2A-Adrenoreceptor in rat hypothalamus
    Immunohistochemical staining of rat ventromedial hypothalamus free floating frozen section using Anti-α2A-Adrenergic Receptor (extracellular) Antibody (#AAR-020), (1:250). A. α2A-Adrenoreceptor staining (green) appears in astrocytic fibers (arrows) originating from the wall of the 3rd ventricle (3rd V). B. DAPI is used as the counterstain (blue). C. Merged image of panels A and B.
Indirect flow cytometry
  • Cell surface detection of α2A-Adrenergic Receptor by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells:
    Cell surface detection of α2A-Adrenergic Receptor by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-α2A-Adrenergic Receptor (extracellular) Antibody (#AAR-020), (5μg) + goat-anti-rabbit-FITC.
References
  1. IUPHAR RECEPTOR DATABASE | ADRENOCEPTORS
  2. Piascik, M.T. and Perez, D.M. (2001) J. Pharmacol. Exp. Ther. 298, 403.
  3. Civantos Calzada, B. and Aleixandre de Artinano, A. (2001) Pharmacol. Res. 44, 195.
  4. Bylund, D.B. (2005) Br. J. Pharmacol. 144, 159.
Scientific background

Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.
 
Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular N-terminus, and a cytoplasmic C- terminus.

Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2. Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2.
 
In general, the pharmacological properties of each subtype are quite homogenous across different species. However, this is not the case for the α2A subtype which was first isolated from human platelets3. This subtype shows different pharmacological properties from that of mouse and rat. For this reason, until molecular techniques significantly advanced, it was believed that human α2A and that of mouse and rat (termed α2D) were two different subtypes. Today, it is accepted that these two subtypes are in fact one gene product and is generally termed α2A. One of the main differences between this subtype from the different organisms is its affinity for yohimbine and rauwolscine; rat and mouse α2A displays less affinity compared to human3,4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-α2A-Adrenergic Receptor (extracellular) Antibody (#AAR-020) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize α2A-adrenoceptor from mouse, rat, and human samples.

For research purposes only, not for human use
Shipping and Ordering information