Anti-β2-Adrenergic Receptor (extracellular)-FITC Antibody

Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, B2AR
Cat #: AAR-016-F
Alternative Name Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, B2AR
  • Lyophilized Powder
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m
      • Peptide (C)NGSRAPDHDVTQERDE, corresponding to amino acid residues 15-30 of mouse ADRB2 (Accession P18762). Extracellular, N-terminus.
    Accession (Uniprot) Number P18762
    Gene ID 11555
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human - 15/16 amino acid residues identical; rat - 14/16 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AAR-016), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic, lci
      • Cell surface detection of β2-Adrenoceptor in live intact human THP-1 monocytic leukemia cells:
        ___ Cells alone.
        ___ Cells + Rabbit IgG isotype control-FITC.
        ___ Cells + Anti-β2-Adrenergic Receptor (extracellular)-FITC Antibody (#AAR-016-F), (5 µg/0.5x106 cells).
        Cell surface detection of β2-Adrenoceptor in live intact human Jurkat T-cell leukemia cells:
        ___ Cells alone.
        ___ Cells + Rabbit IgG isotype control-FITC.
        ___ Cells + Anti-β2-Adrenergic Receptor (extracellular)-FITC Antibody (#AAR-016-F), (5 µg/0.5x106 cells).
        The control antigen is not suitable for this application.
      • G-protein coupled receptors (GPCRs) comprise the largest protein superfamily in mammalian genomes. Signal transduction by GPCRs is fundamental for most physiological processes, spanning from vision, smell and taste to neurological, cardiovascular, endocrine, and reproductive functions, thus, making the GPCR superfamily a major target for therapeutic intervention.

        The β2-adrenergic receptor (ADRB2) is comprised of seven transmembrane α-helices, connected by three extracellular loops and three intracellular loops. The extracellular part, responsible for ligand binding, also includes the N-terminus. The intracellular C-terminal interacts with G-proteins, arrestins and other downstream effectors1.

        Unlike some GPCRs, ADRB2 activates more than one G-protein and signals through at least one known G-protein-independent pathway, arrestin. In addition, there is a rich diversity of available ligands for ADRB2. These ligands are often characterized as inverse agonists that suppress basal activity, full agonists that maximally activate the receptor, partial agonists that produce submaximal activity even at saturating concentrations, and neutral antagonists that occupy the orthosteric binding site but do not affect basal activity2.

        ADRB2 located on the surface of cardiomyocytes, mediates distinct effects on cardiac function and the development of heart failure by regulating production of the secondary messenger cyclic adenosine monophosphate (cAMP). In cardiomyocytes from healthy adult rats and mice, spatially confined ADRB2 induces cAMP signals that are localized exclusively to the deep transverse tubules. In contrary, in cardiomyocytes derived from a rat model of chronic heart failure, ADRB2 is redistributed from the deep transverse tubules to the cell crest, which leads to diffuse receptor-mediated cAMP signaling. Thus, the redistribution of ADRB2 in heart failure might contribute to the failing myocardial phenotype3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 14/07/2019

    Anti-β2-Adrenergic Receptor (extracellular) Antibody (#AAR-016) is a highly specific antibody directed against an extracellular epitope of the mouse β2-adrenoceptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize β2AR from mouse, rat, and human samples.

    Anti-β2-Adrenergic Receptor (extracellular)-FITC Antibody (#AAR-016-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

    For research purposes only, not for human use