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Anti-β1-Adrenergic Receptor (extracellular)-ATTO Fluor-488 Antibody

Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR

Cat #: AAR-023-AG
Alternative Name Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)RAESDEARR(S)YNDP, corresponding to amino acid residues 200-214 of rat ADRB1 (Accession P18090). 2nd extracellular loop.
Accession (Uniprot) Number P18090
Gene ID 24925
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – 14/15 amino acid residues identical.
RRID AB_2756540.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AAR-023), and immunohistochemistry (labeled antibody).
Applications: if, ih
May also work in: ic*, lci*
Immunohistochemistry
  • Expression of β1-Adrenoceptor in mouse cerebellum
    Expression of β1-Adrenoceptor in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum using Anti-β1-Adrenergic Receptor (extracellular)-ATTO-488 Antibody (#AAR-023-AG), (1:60). β1-adrenoceptor staining (green) appears in granule cells and in the Purkinje cell layer. B. Nuclei staining using DAPI as the counterstain. C. Merged image of panels A and B.
References
  1. Warne, T. et al. (2008) Nature 454, 486.
  2. Conn, P.M. (2013) Methods Enzymol. 520, 118.
  3. Li, X. (2014) J. Cell. Mol. Med. 18, 1334.
  4. Ardestani, P.M. (2017) Neuropharmacology 116, 371.
Scientific background

β-Adrenergic receptors control key physiological functions through signals encoded by catecholamine hormones and neurotransmitters to activate intracellular signaling pathways. These receptors belong to the amine receptor cluster of rhodopsin-like family of G-protein coupled receptors (GPCRs).

The β1 adrenergic receptor (β1AR) is comprised of an extracellular N-terminus, seven membrane spanning loops (TM1-7) and an intracellular C-terminus1.

β1AR couples to the stimulatory G protein Gs and thereby activates adenylyl cyclase. β1AR also couples to the inhibitory G protein Gi and can induce G protein independent signaling. Differential activation of these pathways can be modulated by different ligands2.

β1AR is widely expressed in cardiac myocytes and is regulated by the microRNA (miRNA) let-7e. Following acute myocardial infraction (AMI) in rats, expression of β1AR is significantly up-regulated while the expression of miRNA let-7a (as well as miRNAs c, d, e and i) is notably down-regulated 6 and 24 hours post myocardial infarction. Blocking the up-regulation of β1AR can serve as a novel therapeutic approach for the treatment of AMI-induced arrhythmia3.

β1AR is also implicated in the pathophysiology of Alzheimer’s disease (AD). Administration of a partial β1AR agonist, behavioral deficits in a mouse model of AD are restored and amyloid beta and tau levels decrease as measured by regional immunohistochemistry4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-β1-Adrenergic Receptor (extracellular) Antibody (#AAR-023) is a highly specific antibody directed against an extracellular epitope the rat β1-adrenoceptor. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize β1AR from mouse, rat and human samples.

Anti-β1-Adrenergic Receptor (extracellular)-ATTO Fluor-488 Antibody (#AAR-023-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-β1-Adrenergic Receptor (extracellular)-ATTO Fluor-488 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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