Name: Anti-β1-Adrenergic Receptor (extracellular) Antibody
Brand: Alomone Labs
SKU: AAR-023

Overview

Cat #: AAR-023

Alternative Name Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)RAESDEARR(S)YNDP, corresponding to amino acid residues 200-214 of rat ADRB1 (Accession P18090). 2^nd extracellular loop.

Accession (Uniprot) Number P18090

Gene ID 64508

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, Mouse, human - 14/15 amino acid residues identical.

RRID AB_2340886.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 0.85 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat lung (lanes 3 and 6) membranes:
1-3. Anti-β₁-Adrenergic Receptor (extracellular) Antibody (#AAR-023), (1:200).
4-6. Anti-β₁-Adrenergic Receptor (extracellular) Antibody, preincubated with β1-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR023).

Immunohistochemistry

Expression of β₁-Adrenoceptor in rat and mouse brain
Immunohistochemical staining of rat dorsal raphe nucleus and mouse cerebellum using Anti-β₁-Adrenergic Receptor (extracellular) Antibody (#AAR-023), (1:400). A. β₁-Adrenoceptor staining (red) in rat dorsal raphe nucleus appears in neurons (horizontal arrows). C. β₁-Adrenoceptor in mouse cerebellum staining (red) is detected in granule cells (horizontal arrows) and in the Purkinje cell layer (vertical arrow). B, D. Nuclear staining using DAPI as the counterstain (blue).

Indirect flow cytometry

Cell surface detection of β1-adrenoceptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-β1-Adrenergic Receptor (extracellular) Antibody (#AAR-023), (2.5μg) + goat-anti-rabbit-FITC.

References

Gilbert, C.E. et al. (2014) Int. J. Mol. Sci. 15, 2929.
Woo, A.Y. et al. (2012) Acta Pharmacol. Sin. 33, 335.
Vicco, M.H. et al. (2014) Can. J. Cardio. 30, 332.
Wallukat, G. et al. (2002) Herz 27, 683.
Kamal, F.A. et al. (2014) Circ. Res. 114, 742.

Scientific background

β-Adrenergic receptors (βAR) belong to the superfamily of G-protein coupled receptors (GPCRs). βAR stimulation by the sympathetic nervous system or circulating catecholamines is involved in peripheral blood circulation, metabolic regulation, muscle contraction, and central neural activities. In the heart, acute βAR stimulation serves as the most powerful means to regulate cardiac output in response to a fight-or-flight situation.

There are three βAR subtypes, β1AR, β2AR and β3AR, in cardiac myocytes¹. The receptors consist of seven membrane-spanning domains, three intra- and three extracellular loops, an extracellular N-terminal domain, and an intracellular C-terminal tail².

β1AR is the predominant receptor subtype expressed in human cardiomyocytes. β1AR is also expressed in the kidney³. It has been implicated in adipogenesis and memory formation⁴.

β1AR has been widely connected with heart disease. Both the downregulation and overstimulation of β1AR have been connected to myocyte apoptosis. In addition, chronic heart failure is characterized by abnormalities in β1AR signal transduction. It was demonstrated that heart failure is also inducible in animal models by overexpression of components of the β1AR signal transduction cascade. Transgenic mice overexpressing β1AR develop, with aging, heart failure similar to that observed in dilated cardiomyopathy in humans⁵.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-β₁-Adrenergic Receptor (extracellular) Antibody (#AAR-023) is a highly specific antibody directed against an extracellular epitope the rat β₁-adrenoceptor. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize β1AR from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 06/03/2024

Applications

Citations

Immunohistochemistry citations

Mouse sample:
Hamlett, E.D. et al. (2020) Neurobiol. Dis. 134, 104616.

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Order Summary

Anti-β₁-Adrenergic Receptor (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Applications

Citations

Specifications

Scientific Background

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