Anti-β1-Syntrophin (SNTB1) Antibody

Syntrophin-2, SNT2B1, BSYN2, 59 kDa dystrophin-associated protein A1 basic component 1, DAPA1B, Tax interaction protein 43, TIP-43
    Cat #: APZ-022
    Alternative Name Syntrophin-2, SNT2B1, BSYN2, 59 kDa dystrophin-associated protein A1 basic component 1, DAPA1B, Tax interaction protein 43, TIP-43
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)EQVPESISNQKRGVK, corresponding to amino acid residues 99 -113 of mouse β1-syntrophin (Accession Q99L88). Intracellular, PH1 domain.
    • Anti-β1-Syntrophin (SNTB1) Antibody
    Accession (Uniprot) Number Q99L88
    Gene ID 20649
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human – identical.
    RRID AB_2756777.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    • Anti-β1-Syntrophin (SNTB1) Antibody
      Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
      1,2. Anti-β1-Syntrophin (SNTB1) Antibody (#APZ-022), (1:500).
      3,4. Anti-β1-Syntrophin (SNTB1) Antibody, preincubated with the negative control antigen.
    • Anti-β1-Syntrophin (SNTB1) Antibody
      Western blot analysis of human HL-60 promyelocytic leukemia cell line lysate (lanes 1 and 3) and human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 4):
      1,2. Anti-β1-Syntrophin (SNTB1) Antibody (#APZ-022), (1:500).
      3,4. Anti-β1-Syntrophin (SNTB1) Antibody, preincubated with the negative control antigen.
    References
    1. Bhat, H.F. et al. (2013) Cell. Mol. Life Sci. 70, 2533.
    2. Suzuki, A. et al. (1995) J. Cell Biol. 128, 373.
    Scientific background

    Syntrophins are a family of five membrane associated adaptor proteins with a molecular weight of 58-60 kDa. Adaptor proteins play a crucial role in coordinating signaling events and may act as rescuers in situations where a subversion from normal signaling exists. Syntrophins are characterized by the presence of a PDZ (postsynaptic density protein-95/disc large/zona occludens-1) split PH (pleckstrin homology) domain and a SU (syntrophin unique) domain within their structure.

    β-1 Syntrophin is a 538 amino acid long protein while β-2 is 540 amino acid in length. Both forms are ubiquitously expressed in mammalian tissues however β-1 is the predominant form. β-1 Syntrophin is the basic form of the protein while α-1 is acidic. Like all forms of syntrophin, β-1 syntrophin may exist as a monomer or dimer within the cell. Almost all isoforms of syntrophin bind to each other.

    Syntrophin is required for the proper localization expression and function of NaV1.4 and NaV1.5 Na+ channels. Both channels take part of a multi-protein complex in which dystrophin and syntrophin proteins play an important role in determining their expression levels. NaV1.4 and NaV1.5 C-termini consist of the PDZ domain-binding motif formed by the last three residues. The C-terminus of NaV1.5 thus binds with the dystrophin of DGC and this interaction is mediated by and β-syntrophin (and α-syntrophin) proteins1.

    Syntrophin binds directly to dystrophin at the vicinity of dystrophin’s GPC binding site. Another low affinity binding site is presumed to exist at the COOH-terminal region of dystrophin2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-β1-Syntrophin (SNTB1) Antibody (#APZ-022) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize SNTB1 from human, mouse, and rat samples.

    For research purposes only, not for human use
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