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Anti-α1B-Adrenergic Receptor (extracellular) Antibody

Alpha-1B adrenoceptor, ADA1B, ADRA1B

Cat #: AAR-018
Alternative Name Alpha-1B adrenoceptor, ADA1B, ADRA1B
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
May also work in: m*
  • Peptide (C)KNANFTGPNQTSSNS, corresponding to amino acid residues 21-35 of human α1B-adrenoceptor (Accession P35368). Extracellular, N-terminus.
Accession (Uniprot) Number P35368
Gene ID 147
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 14/15 amino acid residues identical; rat - 13/15 amino acid residues identical.
RRID AB_2039716.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, lci, wb
May also work in: ip*
Western blot
  • Western blot analysis of α1B-Adrenoceptor in rat brain (lanes 1 and 3), rat kidney (lanes 2 and 4) and GH3 cell line (lanes 5 and 6) lysates:
    Western blot analysis of α1B-Adrenoceptor in rat brain (lanes 1 and 3), rat kidney (lanes 2 and 4) and GH3 cell line (lanes 5 and 6) lysates:
    1,2,5. Anti-α1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018), (1:200).
    3,4,6. Anti-α1B-Adrenergic Receptor (extracellular) Antibody, preincubated with α1B-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR018).
  • Expression of α1B-Adrenoceptor in rat cerebellum
    Expression of α1B-Adrenoceptor in rat cerebellum
    Immunohistochemical staining of frozen section of rat cerebellum using Anti-α1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018), (1:100). α1B-Adrenoceptor (red) is detected in Purkinje cells. Hoechst 33342 was used as the counterstain.
Indirect flow cytometry
  • Cell surface detection of α1B-Adrenoceptor in GH3 living cells:
    Cell surface detection of α1B-Adrenoceptor in GH3 living cells:
    ___ Unstained cells.
    ___ Cells + Anti-α1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018), (10 µg/5x105 cells).
  • The control antigen is not suitable for this application.
Live cell imaging / Immunocytochemistry
  • Expression of α1B-Adrenoceptor in GH3 cells
    Expression of α1B-Adrenoceptor in GH3 cells
    Cell surface detection of α1B-Adrenoceptor in living GH3 cells with Anti-α1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). Nuclear staining of cells using the DNA dye Hoechst 33342 (blue).
  2. Piascik, M.T .and Perez, D.M. (2001) J. Pharmacol. Exp. Ther. 298, 403.
  3. Perez, D.M. et al. (1993) Mol. Pharmacol. 44, 784.
  4. Minneman, K.P. (1988) Pharmacol. Rev. 40, 87.
  5. Schwinn, D.A. et al. (2004) Mayo Clin. Proc. 79, 1423.
Scientific background

Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.

Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.

Adrenoceptors are divided into three types: α1, α2 and β adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3.1,2 They are expressed in nearly all peripheral tissues and in the central nervous system.1,2
All α1-adrenoceptors (α1-ARs) activate phospholipases C and A2.3 In addition to mobilizing intracellular calcium, the α1-ARs have also been shown to activate calcium influx via voltage-dependent and -independent calcium channels.4
α1B-Adrenergic receptor populations are greatest in the spleen, kidney, cerebellum, and fetal brain.5

α1B-Adrenoceptor causes contraction of smooth muscle cells and thereby controls vascular tone, blood pressure, and accelerates the development of atherosclerosis.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/01/2023

Anti-α1BAdrenergic Receptor (extracellular) Antibody (#AAR-018) is a highly specific antibody directed against an extracellular epitope of the human α1B-adrenoceptor. The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize α1B-adrenoceptor from human, rat and mouse samples.

For research purposes only, not for human use



Immunohistochemistry citations
  1. Rat trigeminal ganglia sections.
    Benbow, T. et al. (2020) Neuropharmacology 175, 108197.
Immunofluorescence citations
  1. Rat trigeminal ganglia sections.
    Benbow, T. et al. (2020) Neuropharmacology 175, 108197.


Scientific Background

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