Anti-α1D-Adrenergic Receptor (extracellular) Antibody

Alpha-1D adrenoceptor, Alpha-1D adrenoreceptor, ADRA1D
    Cat #: AAR-019
    Alternative Name Alpha-1D adrenoceptor, Alpha-1D adrenoreceptor, ADRA1D
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)EPVPPDERF*SGITEE, corresponding to amino acid residues 231-245 of rat α1D-adrenoceptor with replacement of cysteine 240 (C240) with serine (*S) (Accession P23944). 3rd extracellular loop.
        Anti-α1D-Adrenergic Receptor (extracellular) Antibody
    Accession (Uniprot) Number P23944
    Gene ID 29413
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human, mouse - 14/15 amino acid residues identical.
    RRID AB_10613460.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, lci, wb
    May also work in: ip*
      • Anti-α1D-Adrenergic Receptor (extracellular) Antibody
        Western blot analysis of rat brain stem (lanes 1 and 3), mouse brain (lanes 2 and 4), SH-SY5Y (lanes 5 and 7) and Jurkat (lanes 6 and 8) lysates:
        1,2,5,6. Anti-α1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019), (1:200).
        3,4,7,8. Anti-α1D-Adrenergic Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
      • Anti-α1D-Adrenergic Receptor (extracellular) Antibody
        Expression of α1D-Adrenoceptor in rat heart
        Immunohistochemical staining of rat heart paraffin embedded sections using Anti-α1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019), (1:100). α1D-Adrenoceptor is expressed in cardiomyocytes of the myocardium and in the smooth muscle of the blood vessels (arrows). Hematoxilin is used as the counterstain.
        Anti-α1D-Adrenergic Receptor (extracellular) Antibody
        Expression of α1D-Adrenoceptor in rat neocortex
        Immunohistochemical staining of α1D-adrenoceptor in rat neocortex using Anti-α1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019).  A. Most intense staining of α1D-adrenoceptor (green) appears in apical dendrites (thin horizontal arrows) but also in the soma (thick horizontal arrow). Few cortical interneurons express α1D-adrenoceptor. B. The same section was also stained for parvalbumin and one cell (marked with a vertical arrow) also expresses α1D-adrenoceptor.
      • Anti-α1D-Adrenergic Receptor (extracellular) Antibody
        Cell surface detection of α1D-Adrenoceptor in intact live Jurkat cells:
        ___ Unstained cells.
        ___ Cells + Anti-α1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019), (5-10 µg antibody/0.5x106 cells).
        The control antigen is not suitable for this application.
      • Anti-α1D-Adrenergic Receptor (extracellular) Antibody
        Expression of α1D-Adrenoceptor in rat PC12 cells
        Cell surface detection of α1D-Adrenoceptor in intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-α1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019) (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Merge of A and live view of the cells.
    References
      • Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.
         
        Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.

        Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1Aα1B, α1Dα2Aα2Bα2C, β1β2β31,2. The adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2.
         
        α1-Adrenergic receptors play an important role in the physiological response to epinephrine and norepinephrine, particularly in the cardiovascular system. All three cloned α1 receptors (α1A, α1B, and α1D) couple to Gq/11. While their cellular distribution is clear, their functional role is less so due to a lack of specific agonists/antagonists3.
         
        As these receptors lack specific agonists/antagonists, knock-out (KO) studies in mice have shed significant light on their cellular role. Individual α1-adrenoceptor KO mice do not display physical abnormalities in general. Similar KO studies indicate that all α1 subtypes seem to be involved in the regulation of blood pressure3. Also, studies indicate that α1D-adrenoceptor seems to play an important role in aortic contraction4, renal function, nociception, basal locomotor activity3 as well as bladder contraction5

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/11/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat α1D-adrenergic receptor. Anti-α1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019) can be used in western blot, indirect flow cytometry and immunohistochemical applications. It has been designed to recognize α1D-adrenoceptor from mouse, rat and human samples.

    For research purposes only, not for human use