Anti-β3-Adrenergic Receptor (extracellular) Antibody

Beta-3 adrenoceptor, Beta-3 adrenoreceptor, ADRB3, B3AR
    Cat #: AAR-017
    Alternative Name Beta-3 adrenoceptor, Beta-3 adrenoreceptor, ADRB3, B3AR
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide DAEAQESH*SNPRC, corresponding to amino acid residues 180-192 of mouse ADRB3 with replacement of cysteine 186 (C186) with serine (*S) (Accession P25962). 2nd extracellular loop.
    • Anti-β3-Adrenergic Receptor (extracellular) Antibody
    Accession (Uniprot) Number P25962
    Gene ID 11556
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.
    RRID AB_2039720.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.75 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*, lci*
    Western blot
    • Anti-β3-Adrenergic Receptor (extracellular) Antibody
      Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
      1,3. Anti-β3-Adrenergic Receptor (extracellular) Antibody (#AAR-017), (1:200).
      2,4. Anti-β3-Adrenergic Receptor (extracellular) Antibody, preincubated with the negative control antigen.
    • Human detrusor lysate (Silva, I. et al. (2020) Br. J. Pharmacol. 177, 1589.).
    Immunohistochemistry
    • Anti-β3-Adrenergic Receptor (extracellular) Antibody
      Expression of β3-Adrenoceptor in rat hippocampus
      Immunohistochemical staining of rat hippocampal dentate gyrus using Anti-β3-Adrenergic Receptor (extracellular) Antibody (#AAR-017), (1:100), (green). β3-Adrenoceptor is strongly expressed in the hilus (H) and in the outer molecular layer (arrows). The distribution overlaps the entire layers, but is not restricted to nerve cells (stained red with mouse anti-Parvalbumin). DAPI is used as the counterstain (blue).
    References
    1. IUPHAR RECEPTOR DATABASE | ADRENOCEPTORS
    2. Piascik, M.T. and Perez, D.M. (2001) J. Pharmacol. Exp. Ther. 298, 403.
    3. Evans, B.A. et al. (1999) Br. J. Pharmacol. 127, 1525.
    4. Klein, J. et al (1999) J. Biol. Chem. 274, 34795.
    Scientific background

    Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.

    Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.

    Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2 Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system.1,2
     
    The β3-adrenoceptor is insensitive to the commonly used β-antagonists and has often been referred to as the 'atypical' β-adrenoceptor. Mouse β3-adrenoceptor has two known splice variants; isoforms β3a-adrenoceptor and β3b-adrenoceptor. Both isoforms are expressed in white and brown adipose tissues.3 The β3b-adrenoceptor is highly expressed in brain.

    The β3- adrenoceptor has been found to play a key role in the lipolytic action of catecholamines.4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 29/06/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the mouse β3-adrenoceptor. Anti-β3-Adrenergic Receptor (extracellular) Antibody (#AAR-017) can be used in western blot and immunohistochemical applications. It has been designed to recognize both splice variants of the β3AR from mouse, rat and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Human and rat detrusor lysate.
      Silva, I. et al. (2020) Br. J. Pharmacol. 177, 1589.
    Immunohistochemistry citations
    1. Human and rat detrusor sections.
      Silva, I. et al. (2020) Br. J. Pharmacol. 177, 1589.
    2. Rat gastric corpus sections (1:200).
      Song, J. et al. (2014) Transl. Res. 164, 22.
    Immunofluorescence citations
    1. Human and rat detrusor sections.
      Silva, I. et al. (2020) Br. J. Pharmacol. 177, 1589.
    2. Rat gastric corpus sections (1:200).
      Song, J. et al. (2014) Transl. Res. 164, 22.
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